Effect of green synthesized nano-titanium synthesized from Trachyspermum ammi extract on seed germination of Vigna radiate

Nisha Elizabeth Sunny; Sneha Susan Mathew; S Venkat Kumar; Panchamoorthy Saravanan; R Rajeshkannan; M Rajasimman; Yasser Vasseghian

doi:10.1016/j.chemosphere.2022.134600

Effect of green synthesized nano-titanium synthesized from Trachyspermum ammi extract on seed germination of Vigna radiate

Chemosphere. 2022 Aug:300:134600. doi: 10.1016/j.chemosphere.2022.134600. Epub 2022 Apr 12.

Authors

Nisha Elizabeth Sunny¹, Sneha Susan Mathew¹, S Venkat Kumar², Panchamoorthy Saravanan³, R Rajeshkannan⁴, M Rajasimman⁴, Yasser Vasseghian⁵

Affiliations

¹ Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT), Vellore, 632014, India.
² Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT), Vellore, 632014, India. Electronic address: venkatkumars@vit.ac.in.
³ Department of Petro Chemical Technology, University College of Engineering-BIT Campus, Anna University, Tiruchirappalli, 620 024, India.
⁴ Department of Chemical Engineering, Annamalai University, Annamalai Nagar, Chidambaram, India.
⁵ Department of Chemistry, Soongsil University, Seoul, 06978, South Korea; The University of Johannesburg, Department of Chemical Engineering, P.O. Box 17011, Doornfontein, 2088, South Africa; Department of Chemical Engineering, Quchan University of Technology, Quchan, Iran.

PMID: 35427654
DOI: 10.1016/j.chemosphere.2022.134600

Abstract

The current work investigates the conditional influence on Vigna radiate seed germination in vitro and in vivo using the green chemistry approach for the manufacture of titanium dioxide nanoparticles (TiO₂ NPs) from seed extract of Trachyspermum ammi (T. ammi). Ultraviolet spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), and X-ray diffraction (XRD) were used to analyze the TiO₂ NPs produced. The crystalline nature of TiO₂ NP was revealed by XRD data, and TEM investigation revealed an irregularity in TiO₂ NP shape with a size of 17.5 nm. UV absorbance at 315 nm for the TiO₂ NPs was observed using Ultraviolet-visible spectrophotometer. The antioxidant potential of the synthesized nanoparticle was discovered to be good. In case of seed germination studies, six concentrations (25, 50 100, 150, 200, and 250 μg mL^{- 1}) of TiO₂ NPs were examined along with the control on Vigna radiata seeds. Germination parameters such as seed vigor index (SVI), germination percentage (GP), germination value (GV) root length (RL) and shoot length (SL) of the Vigna radiata seedlings were observed and results revealed that the green synthesized TiO₂ NPs were significantly improved. The results indicated that the TiO₂ NP affected the plant growth more specifically at lower concentration (50 μg mL^-1) of TiO₂ NPs. Overall, the findings of this present study stipulated that the green TiO₂ NP production can enhance the growth of Vigna radiate under in vitro and in vivo conditions.

Keywords: Antioxidant; Germination; Green synthesis; Nanoparticles; Seeds; Titanium dioxide.

MeSH terms

Ammi*
Germination
Metal Nanoparticles* / chemistry
Plant Extracts / chemistry
Plant Extracts / pharmacology
Seeds
Spectroscopy, Fourier Transform Infrared
Titanium / chemistry
Titanium / pharmacology
Vigna*
X-Ray Diffraction

Substances

Plant Extracts
Titanium