Bisexual individuals are invisible sexual minorities, who face prejudice and "double discrimination" from both heterosexual and sexual minority communities. Despite this, little empirical research has examined bisexual prejudice reduction. To address this, the current study tested an E-contact intervention - a highly structured, text-based, online interaction tool - to reduce bisexual prejudice. Heterosexual participants (N = 170) were randomly allocated to an intergroup (E-contact) or intragroup (control) interaction, with either a male or female interaction partner. Findings indicated that intergroup E-contact resulted in less intergroup anxiety compared to an intragroup interaction, and that this effect was more pronounced when the bisexual interaction partner was male for heterosexual men, but not for heterosexual women. Although E-contact had no direct effect on tolerance toward bisexual individuals or the perceived stability of bisexuality as a sexual orientation, findings revealed that reduced intergroup anxiety mediated the effect of E-contact on these outcome variables. This result was found in the intergroup dynamic of heterosexual men interacting with bisexual men, providing an important experimental demonstration of the potential for harnessing an E-contact intervention to reduce affective prejudice toward bisexual individuals.