Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling

Noriko Sato; Lawrence P Szajek; Peter L Choyke

doi:10.1007/978-1-0716-2160-8_11

Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

Methods Mol Biol. 2022:2463:153-161. doi: 10.1007/978-1-0716-2160-8_11.

Authors

Noriko Sato¹, Lawrence P Szajek², Peter L Choyke³

Affiliations

¹ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. saton@mail.nih.gov.
² PET Department, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
³ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

PMID: 35344173
DOI: 10.1007/978-1-0716-2160-8_11

Abstract

A ⁸⁹Zr-oxine ex vivo cell labeling method for tracking various cells by positron emission tomography (PET) imaging has recently been developed. ⁸⁹Zr-oxine is synthesized from oxine and ⁸⁹Zr-chloride, which was converted from ⁸⁹Zr-oxalate, with neutralization. To track migration of natural killer (NK) cells in vivo in real time by PET imaging, NK cells are labeled with ⁸⁹Zr-oxine ex vivo and infused to a recipient. The labeling is performed by mixing ⁸⁹Zr-oxine solution to NK cell suspension at room temperature, followed by washing. Care should be taken to label the cells at optimal radioactivity doses that maintain their viability and functionality. ⁸⁹Zr-oxine labeled NK cells can be tracked for their migration and distribution by PET/computed tomography imaging for at least 7 days. Of note, this protocol is applicable to other types of cells.

Keywords: 89Zr-oxine; Cell tracking; Natural killer cells; Positron emission tomography; Zirconium-89.

MeSH terms

Killer Cells, Natural
Oxyquinoline*
Positron Emission Tomography Computed Tomography
Positron-Emission Tomography / methods
Zirconium*

Substances

Oxyquinoline
Zirconium