Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice

Xi Gu; Xinde Hu; Daqi Wang; Zhijiao Xu; Fang Wang; Di Li; Geng-Lin Li; Hui Yang; Huawei Li; Erwei Zuo; Yilai Shu

doi:10.1038/s41422-022-00624-y

Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice

Cell Res. 2022 Jul;32(7):699-702. doi: 10.1038/s41422-022-00624-y. Epub 2022 Feb 23.

Authors

Xi Gu^#^{1

2

3

4}, Xinde Hu^#⁵, Daqi Wang^#^{1

2

4}, Zhijiao Xu^{1

2

4}, Fang Wang^{1

2

4}, Di Li^{6

7}, Geng-Lin Li^{1

2

4}, Hui Yang⁵, Huawei Li^{8

9

10

11}, Erwei Zuo¹², Yilai Shu^{13

14

15}

Affiliations

¹ ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
² Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
³ Department of Otolaryngology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
⁴ NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, China.
⁵ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
⁶ State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources, Guangxi University, Nanning, China.
⁷ Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
⁸ ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China. hwli@shmu.edu.cn.
⁹ Institutes of Biomedical Sciences, Fudan University, Shanghai, China. hwli@shmu.edu.cn.
¹⁰ NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, China. hwli@shmu.edu.cn.
¹¹ The Institutes of Brain Science and the Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China. hwli@shmu.edu.cn.
¹² Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. erweizuo@163.com.
¹³ ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China. yilai_shu@fudan.edu.cn.
¹⁴ Institutes of Biomedical Sciences, Fudan University, Shanghai, China. yilai_shu@fudan.edu.cn.
¹⁵ NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, China. yilai_shu@fudan.edu.cn.

^# Contributed equally.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Associated Protein 9 / metabolism
CRISPR-Cas Systems* / genetics
Gene Editing
Hearing Loss* / genetics
Hearing Loss* / therapy
Mice

Substances

CRISPR-Associated Protein 9