SOX8 Affects Tumoral SPARC Expression by Regulating EZH2 to Attenuate Effectiveness of albumin-bound paclitaxel in PDAC

Int J Biol Sci. 2022 Jan 1;18(3):911-922. doi: 10.7150/ijbs.64752. eCollection 2022.

Abstract

Pancreatic cancer is a dismal malignancy with poor prognosis. In spite of progress in surgical technology, chemotherapy is still the cornerstone in the multi-disciplinary treatment. Albumin-bound paclitaxel is a first-line treatment for PDAC patients. Yet the response rate of the drug is far from satisfying. SOX8 is a member of the sex determining region Y-boxes family, which is potentially related to the chemoresistance of tumor. Patient with high expression of SOX8 were insensitive to albumin-bound paclitaxel. SOX8 reduced apoptosis and G2/M cell cycle arrest caused by albumin-bound paclitaxel. SOX8 transcriptionally regulated EZH2, which reduced expression of SPARC by promoting the methylation of SPARC, thereby reducing the transport of albumin-bound paclitaxel in pancreatic cancer cells. EZH2 inhibitor, UNC1999, can reverse the effect of SOX8 on chemo-resistance of albumin-bound paclitaxel. Collectively, our data revealed SOX8/EZH2/SPARC signaling induced primary chemo-resistance of albumin-bound paclitaxel in pancreatic ductal adenocarcinoma.

Keywords: EZH2; PDAC; SOX8; SPARC; albumin-bound paclitaxel; chemo-resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumin-Bound Paclitaxel / metabolism
  • Albumin-Bound Paclitaxel / pharmacology
  • Albumin-Bound Paclitaxel / therapeutic use
  • Apoptosis / genetics
  • Carcinoma, Pancreatic Ductal* / drug therapy
  • Carcinoma, Pancreatic Ductal* / genetics
  • Carcinoma, Pancreatic Ductal* / pathology
  • Enhancer of Zeste Homolog 2 Protein / genetics
  • Enhancer of Zeste Homolog 2 Protein / metabolism
  • Humans
  • Osteonectin / genetics
  • Osteonectin / pharmacology
  • Osteonectin / therapeutic use
  • Paclitaxel / pharmacology
  • Paclitaxel / therapeutic use
  • Pancreatic Neoplasms* / metabolism
  • SOXE Transcription Factors / metabolism

Substances

  • Albumin-Bound Paclitaxel
  • Osteonectin
  • SOX8 protein, human
  • SOXE Transcription Factors
  • SPARC protein, human
  • EZH2 protein, human
  • Enhancer of Zeste Homolog 2 Protein
  • Paclitaxel