A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing

Macarena Moronta Gines; Kerstin S Wendt

doi:10.1007/978-1-0716-2140-0_7

A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing

Methods Mol Biol. 2022:2458:113-122. doi: 10.1007/978-1-0716-2140-0_7.

Authors

Macarena Moronta Gines¹, Kerstin S Wendt²

Affiliations

¹ Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
² Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands. k.wendt@erasmusmc.nl.

PMID: 35103965
DOI: 10.1007/978-1-0716-2140-0_7

Abstract

The investigation of cohesin binding sites throughout different mammalian genomes by ChIP-sequencing has been fundamental to discover how cohesin and CTCF collaborate to form chromatin loops and to gain insight in the intricate regulation of cohesin. Here we describe a detailed ChIP protocol that has been successfully used for different cohesin subunits and cohesin regulators in various cell lines.

Keywords: ChIP; Chromatin immunoprecipitation; Cohesin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding Sites
CCCTC-Binding Factor / genetics
CCCTC-Binding Factor / metabolism
Cell Cycle Proteins / metabolism
Chromatin*
Chromosomal Proteins, Non-Histone* / metabolism
Cohesins
Mammals / metabolism

Substances

CCCTC-Binding Factor
Cell Cycle Proteins
Chromatin
Chromosomal Proteins, Non-Histone