Protocol for deep brain stimulation in the fimbria-fornix of freely moving mice

STAR Protoc. 2021 Dec 21;3(1):101054. doi: 10.1016/j.xpro.2021.101054. eCollection 2022 Mar 18.

Abstract

Deep brain stimulation (DBS) entails neurosurgery to implant electrodes in specific brain structures to modulate the behavior of a particular neural circuit. DBS is best known for treating advanced Parkinson disease and can potentially be applicable to other motor and even cognitive dysfunctions. Here, we describe a detailed protocol allowing for electrode preparation, surgical procedures, stimulation delivery, and field potential recordings in both anesthetized and behaving mice, and the benefit evaluation of DBS at the fimbria-fornix by using a fear conditioning test. For complete details on the use and execution of this protocol, please refer to Hao et al. (2015).

Keywords: Behavior; Cognitive Neuroscience; Model Organisms; Neuroscience.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Deep Brain Stimulation* / methods
  • Electrodes
  • Fear
  • Fornix, Brain / physiology
  • Mice
  • Parkinson Disease* / therapy