Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.
Keywords: Automation; Cell-free protein synthesis; Droplet printing; High throughput; Low-volume liquid handling; Microfluidic adaptive printing; Protein screening.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.