Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity

Dansi Huang; Chao Li; Qing Chen; Xing Xie; Xiong Fu; Chun Chen; Qiang Huang; Zebo Huang; Hao Dong

doi:10.1016/j.foodchem.2021.131922

Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity

Food Chem. 2022 May 30:377:131922. doi: 10.1016/j.foodchem.2021.131922. Epub 2021 Dec 29.

Authors

Dansi Huang¹, Chao Li², Qing Chen³, Xing Xie¹, Xiong Fu¹, Chun Chen¹, Qiang Huang¹, Zebo Huang¹, Hao Dong⁴

Affiliations

¹ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
² SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China. Electronic address: felichao@scut.edu.cn.
³ School of Food Science and Health Preserving, Guangzhou City Polytechnic, Guangzhou 510405, China. Electronic address: chenqing28532@163.com.
⁴ College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

PMID: 34979396
DOI: 10.1016/j.foodchem.2021.131922

Abstract

Rosa roxburghii Tratt pomace (RRTP) has increasingly attracted attention due to its various nutritional ingredients and health benefits. In this study, the free phenolic fraction (RRTP-FPF) and bound phenolic fraction (RRTP-BPF) were extracted from RRTP by solvent extraction method and alkaline hydrolysis method, respectively. The composition of polyphenols in RRTP-FPF and RRTP-BPF were identified by ultra-high performance liquid chromatography equipped with an electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS/MS). In vitro antioxidant assays indicated that RRTP-FPF and RRTP-BPF could scavenge radicals in a dose-dependent manner, and RRTP-BPF exhibited better scavenging activity than RRTP-FPF. In addition, RRTP-FPF and RRTP-BPF (20 ∼ 100 μg/mL) treatment for 24 h could significantly increase the survival rate and decrease reactive oxygen species (ROS) level of paraquat-exposed nematodes through improving the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that RRTP could be as a good and cheap source of natural antioxidants.

Keywords: Antioxidant activity; Caenorhabditis elegans; Phenolics; Pomace; Rosa roxburghii.

MeSH terms

Animals
Antioxidants* / pharmacology
Chromatography, High Pressure Liquid
Nematoda / drug effects
Plant Extracts / pharmacology
Polyphenols* / pharmacology
Rosa* / chemistry
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry

Substances

Antioxidants
Plant Extracts
Polyphenols