A census of the lung: CellCards from LungMAP

Xin Sun; Anne-Karina Perl; Rongbo Li; Sheila M Bell; Eniko Sajti; Vladimir V Kalinichenko; Tanya V Kalin; Ravi S Misra; Hitesh Deshmukh; Geremy Clair; Jennifer Kyle; Laura E Crotty Alexander; Jorge A Masso-Silva; Joseph A Kitzmiller; Kathryn A Wikenheiser-Brokamp; Gail Deutsch; Minzhe Guo; Yina Du; Michael P Morley; Michael J Valdez; Haoze V Yu; Kang Jin; Eric E Bardes; Jarod A Zepp; Terren Neithamer; Maria C Basil; William J Zacharias; Jamie Verheyden; Randee Young; Gautam Bandyopadhyay; Sara Lin; Charles Ansong; Joshua Adkins; Nathan Salomonis; Bruce J Aronow; Yan Xu; Gloria Pryhuber; Jeff Whitsett; Edward E Morrisey; NHLBI LungMAP Consortium

doi:10.1016/j.devcel.2021.11.007

A census of the lung: CellCards from LungMAP

Dev Cell. 2022 Jan 10;57(1):112-145.e2. doi: 10.1016/j.devcel.2021.11.007. Epub 2021 Dec 21.

Authors

Xin Sun¹, Anne-Karina Perl², Rongbo Li³, Sheila M Bell⁴, Eniko Sajti³, Vladimir V Kalinichenko⁵, Tanya V Kalin², Ravi S Misra⁶, Hitesh Deshmukh², Geremy Clair⁷, Jennifer Kyle⁷, Laura E Crotty Alexander⁸, Jorge A Masso-Silva⁸, Joseph A Kitzmiller⁴, Kathryn A Wikenheiser-Brokamp⁹, Gail Deutsch¹⁰, Minzhe Guo², Yina Du⁴, Michael P Morley¹¹, Michael J Valdez³, Haoze V Yu³, Kang Jin¹², Eric E Bardes¹², Jarod A Zepp¹³, Terren Neithamer¹¹, Maria C Basil¹¹, William J Zacharias¹⁴, Jamie Verheyden³, Randee Young³, Gautam Bandyopadhyay⁶, Sara Lin¹⁵, Charles Ansong⁷, Joshua Adkins⁷, Nathan Salomonis¹⁶, Bruce J Aronow¹², Yan Xu², Gloria Pryhuber⁶, Jeff Whitsett², Edward E Morrisey¹⁷; NHLBI LungMAP Consortium

Affiliations

¹ Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Department of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Electronic address: xinsun@health.ucsd.edu.
² Division of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA.
³ Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
⁴ Division of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
⁵ Division of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA; Center for Lung Regenerative Medicine, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
⁶ Department of Pediatrics Division of Neonatology, The University of Rochester Medical Center, Rochester, NY 14642, USA.
⁷ Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA.
⁸ Deparment of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
⁹ Division of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Division of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Department of Pathology & Laboratory Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA.
¹⁰ Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA; Department of Laboratories, Seattle Children's Hospital, OC.8.720, 4800 Sand Point Way Northeast, Seattle, WA 98105, USA.
¹¹ Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
¹² Departments of Biomedical Informatics, Developmental Biology, and Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
¹³ Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
¹⁴ Division of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Department of Internal Medicine, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA.
¹⁵ National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
¹⁶ Department of Pediatrics, University of Cincinnati College of Medicine, 3230 Eden Avenue, Cincinnati, OH 45267, USA; Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
¹⁷ Penn-CHOP Lung Biology Institute, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: emorrise@pennmedicine.upenn.edu.

Abstract

The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing and genetic lineage tracing have revealed novel cell types and enriched functional properties of existing cell types in lung. The time has come to take a new census. Initiated by members of the NHLBI-funded LungMAP Consortium and aided by experts in the lung biology community, we synthesized current data into a comprehensive and practical cellular census of the lung. Identities of cell types in the normal lung are captured in individual cell cards with delineation of function, markers, developmental lineages, heterogeneity, regenerative potential, disease links, and key experimental tools. This publication will serve as the starting point of a live, up-to-date guide for lung research at https://www.lungmap.net/cell-cards/. We hope that Lung CellCards will promote the community-wide effort to establish, maintain, and restore respiratory health.

Keywords: COVID; CellCards; LungMAP; cell cards; disease; lung; regeneration; repair; resource; single cell.

Publication types

Dataset
Research Support, N.I.H., Extramural

MeSH terms

Cell Differentiation / genetics
Databases as Topic
Humans
Lung / cytology*
Lung / metabolism
Lung / physiology*
Regeneration / genetics
Single-Cell Analysis / methods

Abstract

Publication types

MeSH terms

Grants and funding