Discovery of Three New Monoterpenoid Indole Alkaloids from the Leaves of Gardneria multiflora and Their Vasorelaxant and AChE Inhibitory Activities

Molecules. 2021 Nov 27;26(23):7191. doi: 10.3390/molecules26237191.

Abstract

Three novel monoterpenoid indole alkaloids gardflorine A (1), gardflorine B (2), and gardflorine C (3) were isolated from the leaves of Gardneria multiflora. Their structures, including absolute configurations, were established on the basis of spectroscopic methods (MS, UV, IR, 1D and 2D NMR) and circular dichroism experiments. All the compounds were evaluated for their vasorelaxant and acetylcholinesterase (AChE) inhibitory activities. Compound 1 exhibited potent vasorelaxant activity, with an EC50 value of 8.7 μM, and compounds 2 and 3 showed moderate acetylcholinesterase (AChE) inhibitory activities, with IC50 values of 26.8 and 29.2 μM, respectively.

Keywords: AChE inhibitory activity; Gardneria multiflora; monoterpenoid indole alkaloid; vasorelaxant activity.

MeSH terms

  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / pharmacology*
  • Circular Dichroism
  • Loganiaceae / chemistry*
  • Plant Leaves / chemistry*
  • Proton Magnetic Resonance Spectroscopy
  • Secologanin Tryptamine Alkaloids / chemistry
  • Secologanin Tryptamine Alkaloids / pharmacology*
  • Vasodilator Agents / chemistry
  • Vasodilator Agents / pharmacology*

Substances

  • Cholinesterase Inhibitors
  • Secologanin Tryptamine Alkaloids
  • Vasodilator Agents