Comparing behavior following binge ethanol in adolescent and adult DBA/2 J mice

Behav Brain Res. 2022 Feb 15:419:113703. doi: 10.1016/j.bbr.2021.113703. Epub 2021 Dec 3.

Abstract

The adolescent brain undergoes maturation in areas critically involved in reward, addiction, and memory. Adolescents consume alcohol more than any other drug, typically in a binge-like manner. While adults also binge on alcohol, the adolescent brain is more susceptible to ethanol-related damages due to its ongoing development, which may result in persistent behavioral and physical changes, including differences in myelination in the frontal cortex. Sex also impacts ethanol metabolism and addiction progression, suggesting females are more sensitive than males. This study addressed memory, sociability, ethanol sensitivity, and myelin gene expression changes due to binge ethanol, sex, and age. DBA/2 J males and females were exposed to intermittent binge ethanol (4 g/kg, i.g.) from postnatal day (PND) 29-42 or as adults from PND 64-77. Age groups were tested for behaviors at the early phase (24 h - 7 days) and late phase (starting 3 weeks) after the last dose. Adult prefrontal cortex was collected at both phases. Adolescent ethanol impaired late phase memory while adult ethanol showed no impairment. Meanwhile, adolescent males showed early phase tolerance to ethanol-induced locomotor activation, while adult females showed tolerance at both phases. Adult-treated mice displayed reductions in social interaction. Adult ethanol decreased Mal expression, a gene involved in myelin integrity, at the early phase. No differences in myelin gene expression were observed at the late phase. Thus, adolescent binge ethanol more severely impacts memory and myelin gene expression compared to adult exposure, while adult mice display ethanol-induced reductions in social interaction and tolerance to ethanol's locomotor activation.

Keywords: Adolescence; Age differences; Ethanol; Memory; Myelin.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Age Factors
  • Animals
  • Behavior, Animal / physiology*
  • Binge Drinking* / complications
  • Binge Drinking* / metabolism
  • Binge Drinking* / physiopathology
  • Central Nervous System Depressants / administration & dosage
  • Central Nervous System Depressants / pharmacology*
  • Disease Models, Animal
  • Ethanol / administration & dosage
  • Ethanol / pharmacology*
  • Female
  • Male
  • Memory Disorders* / etiology
  • Memory Disorders* / metabolism
  • Memory Disorders* / physiopathology
  • Mice
  • Mice, Inbred DBA
  • Prefrontal Cortex* / drug effects
  • Prefrontal Cortex* / metabolism
  • Prefrontal Cortex* / physiopathology
  • Sex Characteristics
  • Social Behavior*
  • Underage Drinking

Substances

  • Central Nervous System Depressants
  • Ethanol