Whole-genome analysis of Nigerian patients with breast cancer reveals ethnic-driven somatic evolution and distinct genomic subtypes

Nat Commun. 2021 Nov 26;12(1):6946. doi: 10.1038/s41467-021-27079-w.

Abstract

Black women across the African diaspora experience more aggressive breast cancer with higher mortality rates than white women of European ancestry. Although inter-ethnic germline variation is known, differential somatic evolution has not been investigated in detail. Analysis of deep whole genomes of 97 breast cancers, with RNA-seq in a subset, from women in Nigeria in comparison with The Cancer Genome Atlas (n = 76) reveal a higher rate of genomic instability and increased intra-tumoral heterogeneity as well as a unique genomic subtype defined by early clonal GATA3 mutations with a 10.5-year younger age at diagnosis. We also find non-coding mutations in bona fide drivers (ZNF217 and SYPL1) and a previously unreported INDEL signature strongly associated with African ancestry proportion, underscoring the need to expand inclusion of diverse populations in biomedical research. Finally, we demonstrate that characterizing tumors for homologous recombination deficiency has significant clinical relevance in stratifying patients for potentially life-saving therapies.

Publication types

  • Observational Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / genetics*
  • Biopsy
  • Black People / ethnology
  • Black People / genetics
  • Breast / pathology
  • Breast Neoplasms / ethnology
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / mortality
  • Breast Neoplasms / pathology
  • Clonal Evolution*
  • DNA Mutational Analysis
  • Female
  • GATA3 Transcription Factor / genetics
  • Genetic Heterogeneity
  • Genomic Instability
  • Germ-Line Mutation
  • Health Status Disparities*
  • Humans
  • Middle Aged
  • Nigeria / epidemiology
  • Nigeria / ethnology
  • RNA-Seq
  • Risk Assessment
  • Synaptophysin / genetics
  • Trans-Activators / genetics
  • Tumor Microenvironment / genetics
  • White People / ethnology
  • White People / genetics
  • Whole Genome Sequencing

Substances

  • Biomarkers, Tumor
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Synaptophysin
  • Trans-Activators
  • ZNF217 protein, human
  • SYPL1 protein, human