A combined strategy of TK1, HE4 and CA125 shows better diagnostic performance than risk of ovarian malignancy algorithm (ROMA) in ovarian carcinoma

Clin Chim Acta. 2022 Jan 1:524:43-50. doi: 10.1016/j.cca.2021.11.018. Epub 2021 Nov 20.

Abstract

Background: The dual marker algorithm Risk of Ovarian Malignancy Algorithm (ROMA) has been widely used in the clinic for the identification of equivocal pelvic masses in ovarian carcinoma. To obtain higher diagnostic efficiency, we created a new diagnostic index, Risk of Ovarian Malignancy Index (ROMI), by combing thymidine kinase 1 (TK1), HE4 and CA125.

Methods: 335 patients with pelvic masses on imaging and 46 healthy controls were enrolled. Serum TK1 was analyzed before further study. ROMI and ROMA were evaluated for diagnostic efficiency.

Results: The level of TK1 was elevated in malignant ovarian tumors compared to benign masses (p < 0.001) and healthy controls (p < 0.001). TK1 expression was positively correlated with stage, intrapelvic metastasis, lymphatic metastasis and distant metastasis (all p values < 0.001). The area under the receiver operating characteristic curve (AUC) of ROMI was higher than that of ROMA for both pre- and postmenopausal women. ROMI had better sensitivity, specificity, accuracy, and positive and negative predictive values than ROMA in diagnosis of all-stage or stage I + II ovarian carcinoma for both pre- and postmenopausal women.

Conclusions: TK1 is a potential biomarker in detection of ovarian carcinoma. ROMI shows better diagnostic performance than ROMA in distinguishing malignant ovarian tumors from benign masses.

Keywords: Algorithm; Ovarian cancer; Risk of Ovarian Malignancy Algorithm; Risk of Ovarian Malignancy Index; Thymidine kinase 1; Tumor markers.

MeSH terms

  • Algorithms
  • CA-125 Antigen / analysis
  • Female
  • Humans
  • Membrane Proteins / analysis
  • Ovarian Neoplasms* / diagnosis
  • Thymidine Kinase / analysis
  • WAP Four-Disulfide Core Domain Protein 2 / analysis

Substances

  • CA-125 Antigen
  • MUC16 protein, human
  • Membrane Proteins
  • WAP Four-Disulfide Core Domain Protein 2
  • WFDC2 protein, human
  • Thymidine Kinase
  • thymidine kinase 1