Usefulness of SARS-CoV-2 antigen test sample as input for SARS-CoV-2 RT-PCR analysis

J Med Virol. 2022 Apr;94(4):1693-1695. doi: 10.1002/jmv.27459. Epub 2021 Nov 22.

Abstract

SARS-CoV-2 rapid detection is of great interest to prevent viral dissemination. In that sense, antigen tests appeared as a very valuable tool to reach this goal. However, it is possible to obtain a negative result in those patients with low viral loads, and consequently, reverse transcription-polymerase chain reaction (RT-PCR) should be performed on samples from patients with a negative antigen test in which there is a strong suspicion of COVID infection. The common diagnostic algorithm involves taking a second sample for RT-PCR testing. This study evaluates the usefulness of the antigen test sample for carrying out RT-PCR analysis when necessary. Results obtained indicate that can be used a unique sample for both antigen test and RT-PCR. This data showed that it is possible to reduce excessive suspected individuals managing and so on increase staff security and patient comfort.

Keywords: COVID-19; RT-PCR; SARS-CoV-2; antigen test.

MeSH terms

  • COVID-19 / diagnosis
  • COVID-19 / virology
  • COVID-19 Nucleic Acid Testing*
  • COVID-19 Serological Testing*
  • Humans
  • SARS-CoV-2 / genetics
  • SARS-CoV-2 / immunology
  • SARS-CoV-2 / isolation & purification*
  • Sensitivity and Specificity
  • Specimen Handling
  • Viral Load