A common polymorphism in the dopamine transporter gene predicts working memory performance and in vivo dopamine integrity in aging

Neuroimage. 2021 Dec 15:245:118707. doi: 10.1016/j.neuroimage.2021.118707. Epub 2021 Nov 3.

Abstract

Dopamine (DA) integrity is suggested as a potential cause of individual differences in working memory (WM) performance among older adults. Still, the principal dopaminergic mechanisms giving rise to WM differences remain unspecified. Here, 61 single-nucleotide polymorphisms, located in or adjacent to various dopamine-related genes, were assessed for their links to WM performance in a sample of 1313 adults aged 61-80 years from the Berlin Aging Study II. Least Absolute Shrinkage and Selection Operator (LASSO) regression was conducted to estimate associations between polymorphisms and WM. Rs40184 in the DA transporter gene, SLC6A3, showed allelic group differences in WM, with T-carriers performing better than C homozygotes (p<0.01). This finding was replicated in an independent sample from the Cognition, Brain, and Aging study (COBRA; baseline: n = 181, ages: 64-68 years; 5-year follow up: n = 129). In COBRA, in vivo DA integrity was measured with 11C-raclopride and positron emission tomography. Notably, WM as well as in vivo DA integrity was higher for rs40184 T-carriers at baseline (p<0.05 for WM and caudate and hippocampal D2-receptor availability) and at the 5-year follow-up (p<0.05 for WM and hippocampal D2 availability). Our findings indicate that individual differences in DA transporter function contribute to differences in WM performance in old age, presumably by regulating DA availability.

Keywords: (11)C-raclopride; Dopamine D2 receptor; Dopamine transporter; Single-nucleotide polymorphisms; Working memory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging / genetics*
  • Alleles
  • Female
  • Hippocampus / diagnostic imaging*
  • Homozygote
  • Humans
  • Male
  • Memory, Short-Term / physiology*
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Positron-Emission Tomography*
  • Raclopride
  • Receptors, Dopamine D2 / genetics*
  • Receptors, Dopamine D2 / metabolism*

Substances

  • Receptors, Dopamine D2
  • Raclopride