Role of clusterin in the regulation of trophoblast development and preeclampsia

Shanshui Zeng; Yue Pan; Fei Liu; Jiaye Yin; Min Jiang; Yan Long; Xueqin Zhao; Gendie E Lash; Hongling Yang

doi:10.1016/j.bbrc.2021.10.064

Role of clusterin in the regulation of trophoblast development and preeclampsia

Biochem Biophys Res Commun. 2021 Oct 30:583:128-134. doi: 10.1016/j.bbrc.2021.10.064. Online ahead of print.

Authors

Shanshui Zeng¹, Yue Pan², Fei Liu¹, Jiaye Yin¹, Min Jiang¹, Yan Long¹, Xueqin Zhao¹, Gendie E Lash³, Hongling Yang⁴

Affiliations

¹ Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China.
² Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China.
³ Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China. Electronic address: gendie.lash@hotmail.com.
⁴ Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China. Electronic address: hlyang62@126.com.

PMID: 34735874
DOI: 10.1016/j.bbrc.2021.10.064

Abstract

Preeclampsia (PE) threatens the safety of mothers and fetuses, and its pathogenesis is still unclear. Our previous study has found the relationship between PE and serum Clusterin (CLU). This study aimed to investigate the role of CLU on PE. Firstly, levels of CLU in serum and placental tissue from PE patients and healthy pregnancies were compared. Then, RNA sequencing, cell counting kit-8, matrigel invasion, cell apoptosis, and angiogenesis assay were performed to evaluate the role of CLU on primary isolation trophoblast cells. We found the expression of CLU was increased before the clinical syndrome occurred, whereas its level was positively related to the severity of PE. CLU significantly inhibited the expression of matrix metalloproteinase-9 and Vimentin and enhanced E-cadherin to inhibit epithelial-mesenchymal transition of trophoblast cells, further reducing its migration and invasion. Our results suggested that CLU may play a role in regulating trophoblast invasion and migration during placental development, which may be one of the risk factors for PE.

Keywords: Clusterin; Epithelial-mesenchymal transition; Invasion; Preeclampsia.