Prolonged epigenomic and synaptic plasticity alterations following single exposure to a psychedelic in mice

Cell Rep. 2021 Oct 19;37(3):109836. doi: 10.1016/j.celrep.2021.109836.

Abstract

Clinical evidence suggests that rapid and sustained antidepressant action can be attained with a single exposure to psychedelics. However, the biological substrates and key mediators of psychedelics' enduring action remain unknown. Here, we show that a single administration of the psychedelic DOI produces fast-acting effects on frontal cortex dendritic spine structure and acceleration of fear extinction via the 5-HT2A receptor. Additionally, a single dose of DOI leads to changes in chromatin organization, particularly at enhancer regions of genes involved in synaptic assembly that stretch for days after the psychedelic exposure. These DOI-induced alterations in the neuronal epigenome overlap with genetic loci associated with schizophrenia, depression, and attention deficit hyperactivity disorder. Together, these data support that epigenomic-driven changes in synaptic plasticity sustain psychedelics' long-lasting antidepressant action but also warn about potential substrate overlap with genetic risks for certain psychiatric conditions.

Keywords: 5-HT2A receptor; GPCR; depression; epigenomics; hallucinogens; psychedelics; psychosis; schizophrenia; serotonin (5-HT); synaptic plasticity.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amphetamines / pharmacology*
  • Animals
  • Behavior, Animal / drug effects
  • Dendritic Spines / drug effects*
  • Dendritic Spines / metabolism
  • Epigenesis, Genetic / drug effects*
  • Epigenome / drug effects*
  • Epigenomics
  • Extinction, Psychological / drug effects
  • Fear / drug effects
  • Frontal Lobe / drug effects*
  • Frontal Lobe / metabolism
  • Hallucinogens / pharmacology*
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neuronal Plasticity / drug effects*
  • Receptor, Serotonin, 5-HT2A / drug effects*
  • Receptor, Serotonin, 5-HT2A / genetics
  • Receptor, Serotonin, 5-HT2A / metabolism
  • Serotonin 5-HT2 Receptor Agonists / pharmacology*
  • Synapses / drug effects*
  • Synapses / metabolism
  • Time Factors

Substances

  • Amphetamines
  • Hallucinogens
  • Htr2a protein, mouse
  • Receptor, Serotonin, 5-HT2A
  • Serotonin 5-HT2 Receptor Agonists
  • 4-iodo-2,5-dimethoxyphenylisopropylamine