A preliminary study investigating the detection of lymphovascular invasion in germ cell tumors of the testis with double staining for OCT4/CD34

Pathol Res Pract. 2021 Nov:227:153637. doi: 10.1016/j.prp.2021.153637. Epub 2021 Sep 29.

Abstract

Lymphovascular invasion (LVI) is a relevant prognostic factor in germ cell tumors of the testis (GCTT) and it has been included in the AJCC staging system. Nevertheless, its histological assessment is challenging, with low/moderate interobserver agreement also among expert uropathologists. Few studies focused on the potential role of immunohistochemistry to solve this critical issue; as result, in current guidelines there is no indication for additional staining to detect this histological feature. In the present study, we investigated the detection of LVI invasion in a small cohort of GCTT with double staining for OCT4/CD34. Although our results need to be validated in larger case series with follow-up data, they suggest as OCT4/CD34 could be a useful tool for the histological assessment of these tumors, helping to identify some histological mimickers of LVI and modifying the pT/stage in a significant percentage of patients.

Keywords: Double staining; Germ cell tumors of the testis; Lymphovascular invasion; OCT4; Staging.

MeSH terms

  • Adult
  • Antigens, CD34 / analysis*
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Embryonal / chemistry*
  • Carcinoma, Embryonal / pathology
  • Humans
  • Immunohistochemistry*
  • Lymphatic Vessels / chemistry*
  • Lymphatic Vessels / pathology
  • Male
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Neoplasms, Complex and Mixed / chemistry*
  • Neoplasms, Complex and Mixed / pathology
  • Neoplasms, Germ Cell and Embryonal / chemistry*
  • Neoplasms, Germ Cell and Embryonal / pathology
  • Octamer Transcription Factor-3 / analysis*
  • Predictive Value of Tests
  • Retrospective Studies
  • Seminoma / chemistry*
  • Seminoma / pathology
  • Testicular Neoplasms / chemistry*
  • Testicular Neoplasms / pathology
  • Young Adult

Substances

  • Antigens, CD34
  • Biomarkers, Tumor
  • Octamer Transcription Factor-3
  • POU5F1 protein, human

Supplementary concepts

  • Testicular Germ Cell Tumor