SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19

Sophie Martin; Christopher Heslan; Gwénaële Jégou; Leif A Eriksson; Matthieu Le Gallo; Vincent Thibault; Eric Chevet; Florence Godey; Tony Avril

doi:10.1016/j.isci.2021.103185

SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19

iScience. 2021 Oct 22;24(10):103185. doi: 10.1016/j.isci.2021.103185. Epub 2021 Sep 29.

Authors

Sophie Martin^{1

2}, Christopher Heslan³, Gwénaële Jégou^{1

2}, Leif A Eriksson⁴, Matthieu Le Gallo^{1

2}, Vincent Thibault³, Eric Chevet^{1

2}, Florence Godey^{1

2}, Tony Avril^{1

2}

Affiliations

¹ Inserm U1242 Oncogenesis Stress Signaling, 35000 Rennes, France.
² Centre Eugène Marquis, 35000 Rennes, France.
³ Department of Virology, CHU Pontchaillou, 35000 Rennes, France.
⁴ Department of Chemistry & Molecular Biology, University of Gothenburg, 41390 Göteborg, Sweden.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has elicited a unique mobilization of the scientific community to develop efficient tools to understand and combat the infection. Like other coronavirae, SARS-CoV-2 hijacks host cell secretory machinery to produce viral proteins that compose the nascent virions; including spike (S), envelope (E), and membrane (M) proteins, the most exposed transmembrane proteins to the host immune system. As antibody response is part of the anti-viral immune arsenal, we investigate the immunogenic potential of S, E, and M using a human cell-based system to mimic membrane insertion and N-glycosylation. Both S and M elicit specific Ig production in patients with SARS-CoV-2. Patients with moderate and severe diseases exhibit elevated Ig responses. Finally, reduced Ig binding was observed with spike G614 compared to D614 variant. Altogether, our assay points toward an unexpected immune response against M and represents a powerful tool to test humoral responses against actively evolving SARS-CoV-2 variants and vaccine effectiveness.

Keywords: Immunology; Virology.