Consumption of Butylated Starch Alleviates the Chronic Restraint Stress-Induced Neurobehavioral and Gut Barrier Deficits Through Reshaping the Gut Microbiota

Front Immunol. 2021 Sep 17:12:755481. doi: 10.3389/fimmu.2021.755481. eCollection 2021.

Abstract

The beneficial effect of short-chain fatty acids (SCFAs) on host health has been well recognized based on the booming knowledge from gut microbiome research. The role of SCFA in influencing psychological function is highlighted in recent years but has not been fully elucidated. In this study, the SCFA-acylated starches were used to accomplish a sizeable intestine-targeted release of the SCFAs, and the neurobehavioral, immunological, and microbial effects were further investigated. Acetylated-, butylated-, and isobutylated-starch could attenuate the depression-like behaviors and excessive corticosterone production in chronically stressed mice. Butylated- starch significantly reduced the colonic permeability via increasing the tight junction proteins (including ZO-1, Claudin, and Occludin) gene expression and reduced the level of the inflammatory cytokines (including IL-1β and IL-6). The butylated starch's neurological and immunological benefits may be derived from the gut microbiome modifications, including normalizing the abundance of certain beneficial microbes (Odoribacter and Oscillibacter) and metabolomic pathways (Tryptophan synthesis and Inositol degradation). The present findings further validate the brain-beneficial effect of butyrate and offer novel guidance for developing novel food or dietary supplements for improving mental health.

Keywords: butyrate; depression; gut barrier; inflammation; starch.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Gut Axis / drug effects*
  • Brain-Gut Axis / physiology
  • Butyrates / pharmacology*
  • Fatty Acids, Volatile / pharmacology*
  • Gastrointestinal Microbiome / drug effects*
  • Gastrointestinal Microbiome / physiology
  • Mice
  • Permeability / drug effects
  • Restraint, Physical
  • Starch / pharmacology*
  • Stress, Psychological / complications
  • Tight Junction Proteins / drug effects

Substances

  • Butyrates
  • Fatty Acids, Volatile
  • Tight Junction Proteins
  • Starch