Second-generation sequencing assistance in the diagnosis of Pasteurella multocida empyema: A case report

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Aug 28;46(8):920-924. doi: 10.11817/j.issn.1672-7347.2021.200589.
[Article in English, Chinese]

Abstract
in English, Chinese

Pasteurella multocida empyema is rare and easy to be misdiagnosed. An 81-year-old male patient showed symptoms with cough, sputum, and fever for 3 days. Community-acquired pneumonia was diagnosed firstly. After anti-infection treatment, the patient was still in fever. Chest radiography showed pleural effusion, closed thoracic drainage was performed and the reddish-brown fluid was drained out. The second-generation sequencing was performed on pleural fluid and Pasteurella multocida was detected. Pasteurella multocida has strict requirements for growth conditions and it difficult to cultivate. The application of second-generation sequencing is helpful to diagnose the pathogen rapidly.

多杀巴斯德菌脓胸少见，容易被漏诊。1例81岁患者因“咳嗽、咳痰、发热3 d”入院，诊断为“社区获得性肺炎”，经抗感染治疗后仍发热，胸X射线摄影检查提示胸腔积液，行胸腔闭式引流，引流出红褐色液体，将胸腔积液行二代测序，检测出多杀巴斯德菌。多杀巴斯德菌对生长条件要求比较严格，是一种难以培养的细菌，而二代测序的应用有助于快速诊断病原体。.

Keywords: Pasteurella multocida; empyema; second-generation sequencing.

Publication types

  • Case Reports

MeSH terms

  • Aged, 80 and over
  • Empyema*
  • Humans
  • Male
  • Pasteurella Infections* / diagnosis
  • Pasteurella multocida* / genetics
  • Pleural Effusion*
  • Sputum