Interactions between Brassica vegetables and human saliva can affect in-mouth odor development, which in turn may be linked to individual perception and liking. S-Methyl-l-cysteine sulfoxide is a unique substrate present in Brassicas that produces odor-active sulfur volatiles due to the activity of enzymes present in plant tissue and due to bacteria, which may be present to varying extents in an individual's oral microbiome. Proton transfer reaction mass spectrometry was applied to measure individual differences in sulfur volatile production in real time when fresh human saliva was incubated ex vivo with raw cauliflower for a cohort of child-adult pairs. Large differences in the rate of sulfur volatile production were measured between individuals, but not between age groups. Significant positive relationships were found for volatile production between the adult-child pairs, suggesting a degree of commonality in saliva composition and oral microbiome activity. Furthermore, significant negative relationships were measured between the amount of in-mouth sulfur volatile production and liking for raw cauliflower in children.
Keywords: Brassica; PTR-MS; S-methyl-l-cysteine sulfoxide; cysteine lyase; olfactometry; saliva.