Locomotor Assay in Drosophila melanogaster

Qingqing Liu; Jingsong Tian; Xing Yang; Yan Li; Aike Guo

doi:10.21769/BioProtoc.2283

Locomotor Assay in Drosophila melanogaster

Bio Protoc. 2017 May 20;7(10):e2283. doi: 10.21769/BioProtoc.2283.

Authors

Qingqing Liu^{1

2}, Jingsong Tian^{1

2}, Xing Yang^{1

3}, Yan Li¹, Aike Guo^{1

3}

Affiliations

¹ State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences (CAS), Beijing, China.
² University of CAS, Beijing, China.
³ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China.

Abstract

This protocol describes a simple locomotor assay in Drosophila melanogaster. In brief, the locomotor of each single fly in the culture dish is recorded by a web camera. The moving time, walking length, speed and the locomotor trails of the single fly could be quantitatively analyzed.

Keywords: Behavior; Drosophila; Locomotor; Motion detection; Motion trail; Video analysis.