Real-world particulate matters induce lung toxicity in rats fed with a high-fat diet: Evidence of histone modifications

Xuejingping Han; Meiping Tian; Pavel V Shliaha; Jie Zhang; Shoufang Jiang; Bingru Nan; Md Nur Alam; Ole N Jensen; Heqing Shen; Qingyu Huang

doi:10.1016/j.jhazmat.2021.126182

Real-world particulate matters induce lung toxicity in rats fed with a high-fat diet: Evidence of histone modifications

J Hazard Mater. 2021 Aug 15:416:126182. doi: 10.1016/j.jhazmat.2021.126182. Epub 2021 May 23.

Authors

Xuejingping Han¹, Meiping Tian², Pavel V Shliaha³, Jie Zhang⁴, Shoufang Jiang⁵, Bingru Nan¹, Md Nur Alam¹, Ole N Jensen⁶, Heqing Shen⁷, Qingyu Huang⁸

Affiliations

¹ Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China.
³ Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
⁴ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, 4221-117 Xiang An Nan Road, Xiamen 361102, China. Electronic address: jie.zhang@xmu.edu.cn.
⁵ Department of Occupational and Environmental Health, School of Public Health, North China University of Science and Technology, Tangshan 063000, China.
⁶ Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. Electronic address: jenseno@bmb.sdu.dk.
⁷ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, 4221-117 Xiang An Nan Road, Xiamen 361102, China.
⁸ Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Electronic address: qyhuang@iue.ac.cn.

PMID: 34492953
DOI: 10.1016/j.jhazmat.2021.126182

Abstract

Exposure to ambient particulate matters (PMs) has been associated with a variety of lung diseases, and high-fat diet (HFD) was reported to exacerbate PM-induced lung dysfunction. However, the underlying mechanisms for the combined effects of HFD and PM on lung functions remain poorly unraveled. By performing a comparative proteomic analysis, the current study investigated the global changes of histone post-translational modifications (PTMs) in rat lung exposed to long-term, real-world PMs. In result, after PM exposure the abundance of four individual histone PTMs (1 down-regulated and 3 up-regulated) and six combinatorial PTMs (1 down-regulated and 5 up-regulated) were significantly altered in HFD-fed rats while only one individual PTM was changed in rats with normal diet (ND) feeding. Histones H3K18ac, H4K8ac and H4K12ac were reported to be associated with DNA damage response, and we found that these PTMs were enhanced by PM in HFD-fed rats. Together with the elevated DNA damage levels in rat lungs following PM and HFD co-exposure, we demonstrate that PM exposure combined with HFD could induce lung injury through altering more histone modifications accompanied by DNA damage. Overall, these ﬁndings will augment our knowledge of the epigenetic mechanisms for pulmonary toxicity caused by ambient PM and HFD exposure.

Keywords: DNA damage; High-fat diet; Histone modifications; Lung toxicity; Real-world PM exposure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diet, High-Fat* / adverse effects
Histone Code
Lung
Lung Injury*
Particulate Matter / toxicity
Protein Processing, Post-Translational
Proteomics
Rats

Substances

Particulate Matter