Amphiphilic Copolymers for Versatile, Facile, and In Situ Tunable Surface Biofunctionalization

Adv Mater. 2021 Oct;33(42):e2102489. doi: 10.1002/adma.202102489. Epub 2021 Aug 25.

Abstract

Precision surface engineering is key to advanced biomaterials. A new platform of PEGylated styrene-maleic acid copolymers for adsorptive surface biofunctionalization is reported. Balanced amphiphilicity renders the copolymers water-soluble but strongly affine for surfaces. Fine-tuning of their molecular architecture provides control over adsorptive anchorage onto specific materials-which is why they are referred to as "anchor polymers" (APs)-and over structural characteristics of the adsorbed layers. Conjugatable with an array of bioactives-including cytokine-complexing glycosaminoglycans, cell-adhesion-mediating peptides and antimicrobials-APs can be applied to customize materials for demanding biotechnologies in uniquely versatile, simple, and robust ways. Moreover, homo- and heterodisplacement of adsorbed APs provide unprecedented means of in situ alteration and renewal of the functionalized surfaces. The related options are exemplified with proof-of-concept experiments of controlled bacterial adhesion, human umbilical vein endothelial cell, and induced pluripotent cell growth on AP-functionalized surfaces.

Keywords: adsorptive surface functionalization; antimicrobial surface properties; cell-instructive properties; styrene-maleic anhydride copolymers.

MeSH terms

  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / pharmacology
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Adhesion / drug effects
  • Cytokines / chemistry
  • Glycosaminoglycans / chemistry
  • Gram-Negative Bacteria / drug effects
  • Gram-Positive Bacteria / drug effects
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism
  • Maleates / chemistry
  • Oligopeptides / chemistry
  • Polyethylene Glycols / chemistry
  • Polymers / chemistry*
  • Polymers / pharmacology
  • Styrene / chemistry
  • Surface Properties

Substances

  • Anti-Infective Agents
  • Biocompatible Materials
  • Cytokines
  • Glycosaminoglycans
  • Maleates
  • Oligopeptides
  • Polymers
  • Polyethylene Glycols
  • Styrene
  • arginyl-glycyl-aspartic acid
  • maleic acid