Objectives: To investigate the expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia (OLK) and OLK cancerization who have aspicy diet in Chengdu.
Methods: Thirtypatients with OLK andspicy diet and 15 patients with OLK without spicy diet in Chengdu were divided into three groups: hyperplastic OLK (OLK-), OLK with mild to moderate dysplasia (OLK+), and severe dysplastic OLK or oral squamous cell carcinoma (OSCC) transforming from OLK (OLK++/OSCC). The expression of Ki-67, Cyclin D1, P53, and P16 were detected by immunohistochemistry and statistically analyzed.
Results: The expression of Ki-67 and P53 in patients with or without spicy diet in the OLK+and OLK++/OSCC groups were stronger than that of the OLK- group (P<0.05). The OLK++/OSCC group showed a higher expression of Cyclin D1 and lower expression of P16 than the OLK- group (P<0.05). The expression of Ki-67, Cyclin D1, P53, and P16 in patients with spicy diet and without spicy diet had no substantial difference. The expression of Ki-67 and Cyclin D1 showed a positive correlation (r=0.439, P=0.015).
Conclusions: Spicy diet did not have an influence on the expression of Ki-67, Cyclin D1, P53, and P16 in patients with OLK and OSCC. The expression of Ki-67, Cyclin D1, and P53 increased with the development of OLK, whereas P16 showed opposite expression trend.
目的: 探讨Ki-67、Cyclin D1、P53和P16在成都地区有辛辣饮食习惯的白斑（OLK）及OLK癌变患者中的表达。方法: 将成都地区有辛辣饮食习惯的30例OLK患者和无辛辣饮食习惯的15例OLK患者，分别分成OLK不伴上皮异常增生（OLK-）、OLK伴轻-中度上皮异常增生（OLK+）、OLK伴重度上皮异常增生或癌变（OLK++/OSCC）3组，采用免疫组化检测Ki-67、Cyclin D1、P53和P16在各组的表达，并对结果进行统计分析。结果: 在有/无辛辣饮食习惯的OLK及癌变患者中，Ki-67和P53在OLK+组和OLK++/OSCC组的表达高于OLK-组（P<0.05），Cyclin D1在OLK++/OSCC组的表达高于OLK-组（P<0.05），P16在OLK++/OSCC组的表达低于OLK-组（P<0.05）。Ki-67、Cyclin D1、P53、P16在有和无辛辣饮食习惯的OLK患者中的表达差异无统计学意义（P>0.05）。Ki-67与Cyclin D1的表达成正相关（r=0.439，P=0.015）。结论: 辛辣饮食习惯对OLK及癌变患者Ki-67、Cyclin D1、P53和P16的表达无影响，但是Ki-67、Cyclin D1和P53表达随OLK病变的进展而升高，P16表达随病变进展而降低。.
Keywords: cancerization; oral leukoplakia; oral potentially malignant disorders; spicy diet.