Cytokines and chemokines expression pattern in herpes simplex virus type-1 encephalitis

Neurosci Lett. 2021 Oct 15:763:136170. doi: 10.1016/j.neulet.2021.136170. Epub 2021 Aug 12.

Abstract

To explore the differently expressed cytokines and chemokines to understand the pathways that lead to herpes simplex encephalitis (HSE). Mice in the experimental group were inoculated intracranially with HSV-1. A high-throughput cytokine chip assay was employed to assess the expression of cytokines/chemokines in the mice brain. GO, KEGG, and PPIs analyses were used to investigate the biological process (BP), pathways and interaction network of the differently expressed proteins (DEPs) in HSE. 13 DEPs and various proteins-related signal pathways were identified in HSE, including three new factors (IL-1α, MIP-1γ, and sTNF RI). The proteins were mainly implicated in leukocyte activation and chemotaxis. Additionally, the DEPs constituted a pivotal protein interaction network where IL-6 might be a mediator. 13 DEPs and a series of related signal pathways were associated with the pathophysiological mechanisms responsible for HSE. IL-6 might be a key mediator in the inflammatory responses to the disease.

Keywords: Bio-informatics analysis; Cytokines/chemokines; Encephalitis; Expression; Herpes simplex virus type-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / immunology
  • Brain / pathology*
  • Brain / virology
  • Chlorocebus aethiops
  • Cytokines / analysis
  • Cytokines / metabolism*
  • Disease Models, Animal
  • Encephalitis, Herpes Simplex / immunology*
  • Encephalitis, Herpes Simplex / pathology
  • Encephalitis, Herpes Simplex / virology
  • Gene Expression Profiling
  • Herpesvirus 1, Human / immunology*
  • Humans
  • Male
  • Mice
  • Protein Interaction Maps / genetics
  • Protein Interaction Maps / immunology
  • Vero Cells

Substances

  • Cytokines