Metabolomics Analysis in Different Development Stages on SP0 Generation of Rice Seeds After Spaceflight

Deyong Zeng; Jie Cui; YiShu Yin; Yi Xiong; Mengyao Liu; Shuanghong Guan; Dayou Cheng; Yeqing Sun; Weihong Lu

doi:10.3389/fpls.2021.700267

Metabolomics Analysis in Different Development Stages on SP0 Generation of Rice Seeds After Spaceflight

Front Plant Sci. 2021 Jun 30:12:700267. doi: 10.3389/fpls.2021.700267. eCollection 2021.

Authors

Deyong Zeng^{1

2}, Jie Cui^{1

2}, YiShu Yin^{1

2}, Yi Xiong^{1

2}, Mengyao Liu^{1

2}, Shuanghong Guan^{1

2}, Dayou Cheng^{1

2}, Yeqing Sun³, Weihong Lu^{1

2}

Affiliations

¹ Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
² National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin, China.
³ Dalian Maritime University, Environmental Systems Biology Institute, Dalian, China.

Abstract

Spaceflight is a special abiotic stress condition. In recent years, it has been confirmed that the spaceflight caused the stress response of rice seeds, and the protein level, transcription level, and methylation level will change during the planting process after returning to the ground. However, the changes at the metabolome level are not very clear. In this study, two kinds of rice seeds, Dongnong423 (DN3) and Dongnong416 (DN6), were carried on the ShiJian-10 retractable satellite (SJ-10) for 12.5 days in orbit, returned to the ground and planted in the field until the three-leaf (TLP) and tillering stage (TS). The results of antioxidant enzyme activity, soluble sugar, and electron leakage rate revealed that the spaceflight caused the stress response of rice. The TLP and TS of DN3 identified 110 and 57 different metabolites, respectively, while the TLP and TS of DN6 identified 104 and 74 different metabolites, respectively. These metabolites included amino acids, sugars, fatty acids, organic acids and secondary metabolites. We used qRT-PCR technology to explore the changes of enzyme genes in the tricarboxylic acid cycle (TCA) and amino acid metabolism pathway. Combined with the results of metabolomics, we determined that during the TLP, the TCA cycle rate of DN3 was inhibited and amino acid metabolism was activated, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. In TS, the TCA cycle rate of DN3 was inhibited, and amino acid metabolism was not significantly changed, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. These results suggested that the response mechanisms of the two different rice strains to spaceflight stress are different, and these differences may be reflected in energy consumption and compound biosynthesis of rice in different growth and development stages. This study provided new insights for further exploring the effects of spaceflight.

Keywords: SJ-10 returning satellite; amino acid metabolism; metabolomics; rice; spaceflight; tricarboxylic acid cycle.