The role of citrulline, intestinal fatty acid-binding protein, and D-dimer as potential biomarkers in the diagnosis of internal herniation after Roux-en-Y gastric bypass

Surg Obes Relat Dis. 2021 Oct;17(10):1704-1712. doi: 10.1016/j.soard.2021.05.028. Epub 2021 May 25.

Abstract

Background: Diagnosing internal herniation (IH) in Roux-en-Y gastric bypass (RYGB) patients with acute abdominal pain poses a diagnostic challenge. Diagnostic laparoscopy is often required for a definitive diagnosis. We hypothesized that intestinal ischemia biomarkers would aid in the diagnosing of IH.

Objectives: To explore intestinal ischemia biomarkers in diagnosing IH.

Setting: University Hospital, Sweden.

Methods: Prospective inclusion of 46 RYGB patients admitted for acute abdominal pain between June 2015 and December 2017. Blood samples for analysis of citrulline, intestinal fatty acid-binding protein (I-FABP), and D-dimer were drawn <72 hours from admission and compared between patients with IH (n = 8), small bowel obstruction (SBO) (n = 5), other specified diagnoses (n = 12), or unspecified abdominal pain (n = 21). Levels of white blood cell count (WBC), C-reactive protein (CRP), and lactate at admission were compared. A prospective pain questionnaire for time of pain onset and level of pain at onset and at admission was analyzed.

Results: None of the investigated biomarkers differed significantly between diagnosis categories. Most patients with IH had normal CRP, WBC, and D-dimer levels while their lactate levels were significantly lower (P = .029) compared with the rest of the cohort. Neither pain level nor pain duration differed between the groups.

Conclusion: This study shows that citrulline, I-FABP, and D-dimer cannot be used to diagnose IH and indicates that CRP, D-dimer, and lactate are rarely elevated by an IH. Furthermore, pain intensity and duration cannot differentiate patients with IH. A diagnostic laparoscopy remains the gold standard to diagnose and rule out an IH.

Keywords: Abdominal pain; Biomarkers; Citrulline; D-dimer; Gastric bypass; Internal herniation; Intestinal fatty acid–binding protein; Lactate.

MeSH terms

  • Abdominal Pain / diagnosis*
  • Abdominal Pain / etiology
  • Biomarkers / blood
  • Citrulline / blood
  • Fatty Acid-Binding Proteins / blood
  • Fibrin Fibrinogen Degradation Products / analysis
  • Gastric Bypass* / adverse effects
  • Humans
  • Laparoscopy
  • Obesity, Morbid* / surgery
  • Postoperative Complications
  • Prospective Studies
  • Retrospective Studies

Substances

  • Biomarkers
  • Fatty Acid-Binding Proteins
  • Fibrin Fibrinogen Degradation Products
  • fibrin fragment D
  • Citrulline