Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia

Ann Clin Microbiol Antimicrob. 2021 Jun 12;20(1):43. doi: 10.1186/s12941-021-00450-x.

Abstract

Background: Studying time-related changes in susceptible pathogens causing healthcare-associated infections (HAIs) is vital in improving local antimicrobial and infection control practices.

Objectives: Describe susceptibility patterns to several antimicrobials in gram-positive and gram-negative pathogens isolated from patients causing HAIs at three private tertiary care hospitals in Saudi Arabia over a 5-year period.

Methods: Data on trends of antimicrobial susceptibility among bacteria causing HAIs events in children and adults at three tertiary private hospitals located in Riyadh and Qassim, Saudi Arabia, were collected retrospectively between 2015 and 2019 using the surveillance data datasets.

Results: Over a 5-year period, 38,624 pathogens caused 17,539 HAI events in 17,566 patients. About 9450 (53.8%) of patients who suffered HAIs were females and the average age was 41.7 ± 14.3 years (78.1% were adults and 21.9% were children). Gram-negative pathogens were 2.3-times more likely to cause HAIs compared to gram-positive bacteria (71.9% vs. 28.1%). The ranking of causative pathogens in decreasing order was: Escherichia coli (38%), Klebsiella species (15.1%), and Staphylococcus aureus (12.6%). Gram-positive isolates were mostly susceptible to linezolid (91.8%) whereas they were resistant to ampicillin (52.6%), cefoxitin (54.2%), and doxycycline (55.9%). Gram-negative isolates were mostly sensitive to tigecycline (95%) whereas they were resistant to cefotaxime (49.5%) and cefixime (59.6%). During the 5 years, there were relatively stable susceptibility patterns to all tested antimicrobials, except for cefotaxime which shown a susceptibility reduction by 41.4%, among Escherichia coli and Klebsiella species. An increase in the susceptibility of Acinetobacter and Enterobacter and Citrobacter species to all studied antimicrobials was observed except for colistin that had a slight sensitivity reduction in 2019 by 4.3% against Acinetobacter species. However, we noted reduced sensitivity of MRSA, CoNS and Enterococcus species to gentamicin; and increased resistance of MRSA to linezolid and vancomycin.

Conclusion: The observed increase in susceptibility of gram-positive and gram-negative bacteria to studied antimicrobials is important; however, reduced sensitivity of MRSA, CoNS and Enterococcus species to gentamicin; and increased resistance of MRSA to linezolid and vancomycin is a serious threat and calls for effective antimicrobial stewardship programs.

Keywords: Antibiotics; Antimicrobials; Gram-negative; Gram-positive; Healthcare-associated infections; Rates; Saudi Arabia; Sensitivity; Susceptibility.

MeSH terms

  • Adult
  • Anti-Bacterial Agents / pharmacology*
  • Colistin
  • Cross Infection / microbiology*
  • Delivery of Health Care*
  • Drug Resistance, Multiple, Bacterial / drug effects
  • Female
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / drug effects*
  • Gram-Positive Bacteria / classification
  • Gram-Positive Bacteria / drug effects*
  • Hospitals*
  • Humans
  • Linezolid
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Retrospective Studies
  • Saudi Arabia / epidemiology
  • Sensitivity and Specificity
  • Tigecycline
  • Vancomycin

Substances

  • Anti-Bacterial Agents
  • Vancomycin
  • Tigecycline
  • Linezolid
  • Colistin