The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Chantelle Pretorius; Nico J Smit; Bjoern C Schaeffner; Courtney A Cook

doi:10.1016/j.ijppaw.2021.04.008

The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Int J Parasitol Parasites Wildl. 2021 Apr 29:15:143-152. doi: 10.1016/j.ijppaw.2021.04.008. eCollection 2021 Aug.

Authors

Chantelle Pretorius¹, Nico J Smit¹, Bjoern C Schaeffner^{1

2}, Courtney A Cook¹

Affiliations

¹ Water Research Group, Unit for Environmental Sciences and Management, Potchefstroom Campus, North-West University, 11 Hoffman Street, Potchefstroom, 2520, South Africa.
² South African Shark Conservancy, Old Harbour, 22 Marine Drive, Hermanus, 7200, South Africa.

Abstract

With over 200 species of sharks reported from South African waters, the potential of discovering new blood parasites is very high. Unfortunately, this remains a poorly explored area of research, particularly in this biogeographical region. To date, only a single trypanosome species, Trypanosoma haploblephari Yeld and Smit, 2006, has been described from elasmobranchs off the coast of South Africa infecting the catsharks Haploblepharus pictus (Müller & Henle) and Haploblepharus edwardsii (Schinz). With only a single trypanosome species described and absence of molecular information, a study was conducted to provide further morphological and molecular information on T. haploblephari, a species considered not to demonstrate any pleomorphism. Thin blood smears were prepared, and blood was collected in molecular-grade ethanol from the caudal vein of two shark species, H. pictus and Poroderma pantherinum (Müller & Henle). Trypanosomes were morphologically described and molecularly characterised based on analysis of fragments of the 18S ribosomal gene. The presence of T. haploblephari in H. pictus was confirmed using the original description based on morphology, type host and locality, which allowed for the molecular characterisation of the species. In addition, this species was found parasitising P. pantherinum, its morphology considerably different in this host species as compared to that in the species of Haploblepharus, demonstrating that T. haploblephari may show extreme pleomorphism. This paper provides both morphological and molecular data for both morphotypes of T. haploblephari, with molecular comparisons to the only two other elasmobranch species of trypanosome for which sequence data is available. To elucidate the relationship of trypanosomes from aquatic hosts in general, more efforts need to be placed on elasmobranchs, as current phylogenetic studies are predominantly focused on trypanosomes infecting freshwater fishes.

Keywords: 18S rRNA; Elasmobranch parasites; Endemic sharks; Marine fish parasites; South Africa; Trypanosoma.