Global Lipidomics Profiling by a High Resolution LC-MS Platform

Thomas Züllig; Martin Trötzmüller; Harald C Köfeler

doi:10.1007/978-1-0716-1410-5_3

Global Lipidomics Profiling by a High Resolution LC-MS Platform

Methods Mol Biol. 2021:2306:39-51. doi: 10.1007/978-1-0716-1410-5_3.

Authors

Thomas Züllig¹, Martin Trötzmüller¹, Harald C Köfeler²

Affiliations

¹ Core Facility Mass Spectrometry, Medical University of Graz, Graz, Austria.
² Core Facility Mass Spectrometry, Medical University of Graz, Graz, Austria. Harald.koefeler@medunigraz.at.

PMID: 33954938
DOI: 10.1007/978-1-0716-1410-5_3

Abstract

Lipidomics is the determination of big lipid assemblies by mass spectrometry. When using chromatography coupled high resolution mass spectrometry, lipids can be identified by exact mass, fragment spectra, and retention time. This protocol covers lipid extraction, LC-MS data acquisition by Orbitrap mass spectrometry and data processing by Lipid Data Analyzer, a custom developed open source software.

Keywords: Chromatography; LC-MS; Lipid data analyzer; Lipidomics; Lipids; Mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Computational Biology
Data Analysis
Lipidomics / methods*
Lipids / analysis*
Software
Tandem Mass Spectrometry

Substances

Lipids