Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

Jun Zhao; Tian Yue; Shibi Lu; Haoye Meng; Qiuxia Lin; Haiyang Ma; Guangbo Liu; Huo Li; Qiang Lu; Aiyuan Wang; Wenjing Xu; Jing Feng; Yiqun Wan; Sida Liao; Xuefeng Zhou; Jiang Peng

doi:10.1016/j.jot.2020.08.005

Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

J Orthop Translat. 2021 Mar 8:27:132-138. doi: 10.1016/j.jot.2020.08.005. eCollection 2021 Mar.

Authors

Jun Zhao^{1

2}, Tian Yue², Shibi Lu², Haoye Meng², Qiuxia Lin², Haiyang Ma^{1

2}, Guangbo Liu^{1

2}, Huo Li², Qiang Lu², Aiyuan Wang², Wenjing Xu², Jing Feng², Yiqun Wan², Sida Liao^{1

2}, Xuefeng Zhou³, Jiang Peng²

Affiliations

¹ Medical School of Chinese PLA, Beijing, 100853, China.
² Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory (No BZ0128), Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, Beijing, 100853, China.
³ Strategic Support Force Medical Center of chinese PLA, AnxiangBeili, Beijing, 100101, China.

Abstract

Background: Osteonecrosis of the femoral head (ONFH) is a refractory disease due to its unclear pathomechanism. Neither conservative treatment nor surgical treatment during the early stage of ONFH achieves satisfactory results. Therefore, this study aims to explore the available evidence on the effect of zoledronic acid on early-stage ONFH.

Methods: For groups were established:the Normal group, model group, Normal saline group(NS group) and zoledronic acid-treated group. The blood supply to the femoral head of animals in the model group and zoledronic acid-treated group was interrupted via a surgical procedure, and zoledronic acid was then locally administered to the femoral head. Four weeks after surgery, all the hips were harvested and evaluated by micro-CT and histopathology(H&E staining, TRAP staining, Toluidine blue staining and masson staining).

Results: The values of BMD, BS/BV and Tb.Th in the Normal group and zoledronic acid-treated group were significantly higher than those in the model group and NS group (p < 0.05). The outcome of H&E staining, Toluidine blue staining and masson staining were consistent with that of micro-CT.

Conclusion: The local administration of zoledronic acid in the femoral head had positive effects on the bone structure of the femoral head in a modified rat model of traumatic ONFH and offered a promising therapeutic strategy during the early stage of ONFH.

The translational potential of this article: This article could provide a choice for treating patients who have osteonecrosis of femora head and can be the basic research for advanced development over this disease.

Keywords: Early stage; Local administration; Osteonecrosis of the femoral head; Prevent; zoledronic acid.