Alginate-based 3D cancer cell culture for therapeutic response modeling

STAR Protoc. 2021 Mar 18;2(2):100391. doi: 10.1016/j.xpro.2021.100391. eCollection 2021 Jun 18.

Abstract

Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017), Lehmann et al. (2016), Liu et al. (2016), and Duval et al. (2011).

Keywords: Cancer; Cell culture; Cell-based assays; High-throughput screening.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Antineoplastic Agents / pharmacology*
  • Calcium Chloride
  • Cell Culture Techniques, Three Dimensional / methods*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Humans
  • Models, Biological

Substances

  • Alginates
  • Antineoplastic Agents
  • Calcium Chloride