In vitro induction of trained immunity in adherent human monocytes

Jorge Domínguez-Andrés; Rob J W Arts; Siroon Bekkering; Harsh Bahrar; Bastiaan A Blok; L Charlotte J de Bree; Mariolina Bruno; Özlem Bulut; Priya A Debisarun; Helga Dijkstra; Jéssica Cristina Dos Santos; Anaísa V Ferreira; Daniela Flores-Gomez; Laszlo A Groh; Inge Grondman; Leonie Helder; Cor Jacobs; Liesbeth Jacobs; Trees Jansen; Gizem Kilic; Viola Klück; Valerie A C M Koeken; Heidi Lemmers; Simone J C F M Moorlag; Vera P Mourits; Jelmer H van Puffelen; Katrin Rabold; Rutger J Röring; Diletta Rosati; Helin Tercan; Julia van Tuijl; Jessica Quintin; Reinout van Crevel; Niels P Riksen; Leo A B Joosten; Mihai G Netea

doi:10.1016/j.xpro.2021.100365

In vitro induction of trained immunity in adherent human monocytes

STAR Protoc. 2021 Feb 24;2(1):100365. doi: 10.1016/j.xpro.2021.100365. eCollection 2021 Mar 19.

Authors

Jorge Domínguez-Andrés^{1

2}, Rob J W Arts^{1

2}, Siroon Bekkering^{1

2}, Harsh Bahrar^{1

2}, Bastiaan A Blok^{1

2}, L Charlotte J de Bree^{1

2}, Mariolina Bruno^{1

2}, Özlem Bulut^{1

2}, Priya A Debisarun^{1

2}, Helga Dijkstra^{1

2}, Jéssica Cristina Dos Santos^{1

2}, Anaísa V Ferreira^{1

2}, Daniela Flores-Gomez^{1

2}, Laszlo A Groh^{1

2}, Inge Grondman^{1

2}, Leonie Helder^{1

2}, Cor Jacobs^{1

2}, Liesbeth Jacobs^{1

2}, Trees Jansen^{1

2}, Gizem Kilic^{1

2}, Viola Klück^{1

2}, Valerie A C M Koeken^{1

2

3}, Heidi Lemmers^{1

2}, Simone J C F M Moorlag^{1

2}, Vera P Mourits^{1

2}, Jelmer H van Puffelen^{1

2}, Katrin Rabold^{1

2}, Rutger J Röring^{1

2}, Diletta Rosati^{1

2}, Helin Tercan^{1

2}, Julia van Tuijl^{1

2}, Jessica Quintin⁴, Reinout van Crevel^{1

2}, Niels P Riksen^{1

2}, Leo A B Joosten^{1

2}, Mihai G Netea^{1

2

5}

Affiliations

¹ Department of Internal Medicine, Radboud University Nijmegen Medical Centre, Geert Grooteplein 8, 6500HB Nijmegen, the Netherlands.
² Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Medical Center, 6525 GA Nijmegen, the Netherlands.
³ Department of Computational Biology for Individualised Infection Medicine, Centre for Individualised Infection Medicine (CiiM) and TWINCORE, The Helmholtz Centre for Infection Research (HZI) and The Hannover Medical School (MHH), Hannover, Germany.
⁴ Immunology of Fungal Infections, Department of Mycology, Institut Pasteur, 75015 Paris, France.
⁵ Department for Genomics & Immunoregulation, Life and Medical Sciences Institute (LIMES), University of Bonn, 53115 Bonn, Germany.

Abstract

A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed "trained immunity." We outline here a protocol to recapitulate this in vitro using adherent monocytes from consecutive isolation of peripheral blood mononuclear cells. The induction of trained immunity and the associated functional reprogramming of monocytes is described in detail using β-glucan (from Candida albicans) and Bacillus Calmette-Guérin as examples. For complete details on the use and execution of this protocol, please refer to Repnik et al. (2003) and Bekkering et al. (2016).

Keywords: Cell biology; Cell isolation; Cell-based assays; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cellular Reprogramming / physiology
Cellular Reprogramming Techniques / methods*
Cytokines / immunology
Humans
Immunity, Innate / immunology*
Leukocytes, Mononuclear / cytology
Leukocytes, Mononuclear / physiology
Monocytes / physiology
Mycobacterium bovis / physiology
beta-Glucans / pharmacology

Substances

Cytokines
beta-Glucans