Generation of integration-free induced pluripotent stem cells from healthy individuals

Cristine Marie Yde Ohki; Leoni Grossmann; Christian Döring; Per Hoffmann; Stefan Herms; Anna Maria Werling; Susanne Walitza; Edna Grünblatt

doi:10.1016/j.scr.2021.102269

Generation of integration-free induced pluripotent stem cells from healthy individuals

Stem Cell Res. 2021 May:53:102269. doi: 10.1016/j.scr.2021.102269. Epub 2021 Feb 24.

Authors

Cristine Marie Yde Ohki¹, Leoni Grossmann¹, Christian Döring¹, Per Hoffmann², Stefan Herms², Anna Maria Werling¹, Susanne Walitza³, Edna Grünblatt⁴

Affiliations

¹ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Zurich, Zurich, Switzerland.
² Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany.
³ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland.
⁴ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland. Electronic address: edna.gruenblatt@kjpd.uzh.ch.

PMID: 33667761
DOI: 10.1016/j.scr.2021.102269

Abstract

Ten human induced pluripotent stem cell (iPSC) lines have been derived from five healthy controls matched to a study including Attention-Deficit Hyperactivity Disorder patients (ADHD). Both female and male children and adolescents aged 6-18 years were recruited. Isolated keratinocyte cells from the participants were reprogrammed into iPSCs using non-integrating Sendai virus to deliver the reprogramming factors Oct3/4, Sox2, Klf4 and cMyc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Attention Deficit Disorder with Hyperactivity*
Cell Differentiation
Cellular Reprogramming
Child
Female
Humans
Induced Pluripotent Stem Cells*
Keratinocytes
Kruppel-Like Factor 4
Male
Sendai virus