Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney

Ming-Zhi Zhang; Raymond C Harris

doi:10.21769/BioProtoc.3364

Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney

Bio Protoc. 2019 Sep 20;9(18):e3364. doi: 10.21769/BioProtoc.3364.

Authors

Ming-Zhi Zhang^{1

2}, Raymond C Harris^{1

2

2}

Affiliations

¹ Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
² Vanderbilt Center for Kidney Disease, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.

Abstract

Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney is of particular importance. The current protocol describes CD8α immunostaining using a Vectastain ABC kit. This protocol works well with most commercially available antibodies, including CD8α antibodies in kidneys of diabetic mice.

Keywords: ABC kit; Biotinylated secondary antibody; CD8α lymphocyte; Diabetic nephropathy; Immunostaining.

Abstract

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