E-cig might cause cell damage of oral mucosa

Lígia Ângelo Tavares Schwarzmeier; Beatriz Serralheiro da Cruz; Camila Cristina Panisello Ferreira; Bruna Fernandes do Carmo Carvalho; Mônica Ghislaine Oliveira Alves; Celina Faig Lima Carta; Jaqueline Ribeiro Scholz; Janete Dias Almeida

doi:10.1016/j.oooo.2020.11.009

E-cig might cause cell damage of oral mucosa

Oral Surg Oral Med Oral Pathol Oral Radiol. 2021 Apr;131(4):435-443. doi: 10.1016/j.oooo.2020.11.009. Epub 2020 Nov 19.

Authors

Lígia Ângelo Tavares Schwarzmeier¹, Beatriz Serralheiro da Cruz¹, Camila Cristina Panisello Ferreira¹, Bruna Fernandes do Carmo Carvalho¹, Mônica Ghislaine Oliveira Alves², Celina Faig Lima Carta¹, Jaqueline Ribeiro Scholz³, Janete Dias Almeida⁴

Affiliations

¹ Department of Biosciences and Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, Brazil.
² School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil; School of Medicine, Universidade Anhembi Morumbi, São José dos Campos. São Paulo.
³ Outpatient Smoking Treatment Program of the Heart Institute (Incor), Faculty of Medicine, University of São Paulo (FMUSP), São Paulo, Brazil.
⁴ Department of Biosciences and Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, Brazil. Electronic address: janete.almeida@unesp.br.

PMID: 33610539
DOI: 10.1016/j.oooo.2020.11.009

Abstract

Objective: The aim of this study was to investigate cytogenetic and cytotoxic damage through the evaluation of micronuclei (MN) and metanuclear anomalies in the oral mucosa of electronic cigarette (e-cig) users.

Study design: The patients were recruited into 4 groups: e-cig users, smokers, former smokers, and nonsmokers (control). The samples were collected by means of exfoliative cytology of the lateral region of the tongue and the floor of the mouth. The smears obtained were fixed and stained by the Feulgen method for investigation of MN and metanuclear anomalies.

Results: A significant difference was observed for MN frequency only between the smoker and control groups. As for metanuclear anomalies, significant differences were observed: karyolysis between: smokers and control, e-cig and control, as well as former smokers; karyorrhexis: between smoker and control; binucleation: between e-cig and former smoker, as well as control; broken eggs: between e-cig and all other groups; nuclear bud: between e-cig and former smokers, as well as control.

Conclusions: E-cig and alcohol users presented genotoxicity and cytotoxicity in the oral mucosa cells. The use of e-cigs and alcohol by former smokers can cause more damage to the cells of the oral mucosa compared to those who have not used e-cigs.

MeSH terms

Alcohol Drinking
DNA Damage
Electronic Nicotine Delivery Systems*
Humans
Mouth Mucosa