The differentiation of colorectal cancer is closely relevant to m6A modification

Biochem Biophys Res Commun. 2021 Mar 26:546:65-73. doi: 10.1016/j.bbrc.2021.02.001. Epub 2021 Feb 8.

Abstract

The occurrence and development of tumors cannot be separated from the influence of differentiation at different stages and levels. Our study found that E-cadherin was significantly increased in cell model induced by sodium butyrate and cell density, while METTL3, METTL16 and WTAP were decreased during the differentiation of cells. In the clinicopathological tissues, E-cadherin was low expressed in poorly differentiated tumor tissues and above three regulators were highly expressed in poorly differentiated tissues. At the levels of clinicopathological differentiation, tissue differentiation and cell differentiation, the result indicated that the poor prognosis of colorectal cancer (CRC) may be closely related to high expression of total m6A level and high expression of METTL3, METTL16 and WTAP.

Keywords: Colorectal cancer; Differentiation; E-cadherin; METTL3; WTAP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / metabolism
  • Butyric Acid / pharmacology
  • Cell Cycle Proteins
  • Cell Differentiation* / drug effects
  • Cell Line, Tumor
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology*
  • Contact Inhibition / drug effects
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Male
  • Methyltransferases
  • Middle Aged
  • Protein Biosynthesis / drug effects
  • RNA Splicing Factors
  • Transcription, Genetic / drug effects

Substances

  • Cell Cycle Proteins
  • RNA Splicing Factors
  • WTAP protein, human
  • Butyric Acid
  • N-methyladenosine
  • METTL16 protein, human
  • Methyltransferases
  • METTL3 protein, human
  • Adenosine