Detection of necroptosis by phospho-MLKL immunohistochemical labeling

Peng He; Tingting Ai; Zhang-Hua Yang; Jianfeng Wu; Jiahuai Han

doi:10.1016/j.xpro.2020.100251

Detection of necroptosis by phospho-MLKL immunohistochemical labeling

STAR Protoc. 2020 Dec 30;2(1):100251. doi: 10.1016/j.xpro.2020.100251. eCollection 2021 Mar 19.

Authors

Peng He¹, Tingting Ai², Zhang-Hua Yang¹, Jianfeng Wu^{2

3}, Jiahuai Han^{1

2}

Affiliations

¹ State Key Laboratory of Cellular Stress Biology, Xiang'an Hospital of Xiamen University, Cancer Research Center of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China.
² Research Unit of Cellular Stress of CAMS, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
³ Laboratory Animal Research Center, Xiamen University, Xiamen, Fujian 361102, China.

Abstract

TNF-induced necroptosis is involved in many physiological and pathological processes. Phospho-MLKL is a hallmark of necroptosis. Cecum is a sensitive organ with extensive necroptosis responses to TNF in vivo. Here, taking advantage of commercially available mouse TNF and easily accessible reagents and materials, we systematically provide a detailed and highly versatile protocol of detecting necroptosis signaling in mouse cecum by immunohistochemical labeling, which can also be used in other tissues or antibodies in immunohistochemical staining. For complete details on the use and execution of this protocol, please refer to Yang et al. (2020) and Chen et al. (2015).

Keywords: Antibody; Cell Biology; Immunology; Model Organisms; Molecular/Chemical Probes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Immunohistochemistry*
Mice
Necroptosis*
Phosphoproteins / metabolism*
Protein Kinases / metabolism*

Substances

Phosphoproteins
MLKL protein, mouse
Protein Kinases