High-throughput drug screening of fine-needle aspiration-derived cancer organoids

Kensey Bergdorf; Courtney Phifer; Vijaya Bharti; David Westover; Joshua Bauer; Anna Vilgelm; Ethan Lee; Vivian Weiss

doi:10.1016/j.xpro.2020.100212

High-throughput drug screening of fine-needle aspiration-derived cancer organoids

STAR Protoc. 2020 Dec 16;1(3):100212. doi: 10.1016/j.xpro.2020.100212. eCollection 2020 Dec 18.

Authors

Kensey Bergdorf¹, Courtney Phifer², Vijaya Bharti³, David Westover^{4

5}, Joshua Bauer^{4

5

6}, Anna Vilgelm^{3

7}, Ethan Lee^{1

6

8}, Vivian Weiss^{1

2

6}

Affiliations

¹ Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA.
² Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
³ Department of Pathology, The Ohio State University, Columbus, OH 43210, USA.
⁴ Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
⁵ Vanderbilt Institute of Chemical Biology- High-Throughput Screening Facility, Vanderbilt University, Nashville, TN 37232, USA.
⁶ Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
⁷ The Ohio State University Comprehensive Cancer Center - Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH 43210, USA.
⁸ Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, USA.

Abstract

Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format. We dissociate organoids into single cells and then plate in a 384-well format for high-throughput drug screening. While this method must be fine-tuned for each individual organoid culture, it offers a format well suited for rapidly screening medium-sized drug/compound libraries (500-5,000 molecules) and generating dose-response curves to measure relative efficacy. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).

Keywords: Cancer; Cell culture; Cell-based Assays; High Throughput Screening; Organoids.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biopsy, Fine-Needle / methods*
Cell Culture Techniques / methods
Drug Evaluation, Preclinical / methods*
Early Detection of Cancer / methods
High-Throughput Screening Assays / methods*
Humans
Neoplasms / metabolism
Organoids / cytology
Organoids / metabolism

Abstract

Publication types

MeSH terms

Grants and funding