Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR

J Clin Microbiol. 2020 Dec 17;59(1):e02369-20. doi: 10.1128/JCM.02369-20. Print 2020 Dec 17.

Authors

Manu Vanaerschot¹, Sabrina A Mann^{1

2}, James T Webber¹, Jack Kamm¹, Sidney M Bell³, John Bell⁴, Si Noon Hong⁵, Minh Phuong Nguyen⁵, Lienna Y Chan¹, Karan D Bhatt¹, Michelle Tan¹, Angela M Detweiler¹, Alex Espinosa⁴, Wesley Wu¹, Joshua Batson¹, David Dynerman¹, Debra A Wadford⁴, Andreas S Puschnik¹, Norma Neff¹, Vida Ahyong¹, Steve Miller², Patrick Ayscue¹, Cristina M Tato¹, Simon Paul⁵, Amy L Kistler¹, Joseph L DeRisi^{1

2}, Emily D Crawford^{6

2}

Affiliations

¹ Chan Zuckerberg Biohub, San Francisco, California, USA.
² University of California, San Francisco, California, USA.
³ Chan Zuckerberg Initiative, Redwood City, California, USA.
⁴ California Department of Public Health, Richmond, California, USA.
⁵ Department of Public Health, Madera, California, USA.
⁶ Chan Zuckerberg Biohub, San Francisco, California, USA emily.crawford@ucsf.edu.

No abstract available

Keywords: COVID-19; PCR; SARS-CoV-2; diagnosis; mutation; polymorphism; sensitivity.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution / genetics
COVID-19 / diagnosis*
COVID-19 Testing / methods*
Coronavirus Nucleocapsid Proteins / genetics*
Humans
Polymorphism, Single Nucleotide / genetics*
RNA, Viral / genetics
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2 / genetics
SARS-CoV-2 / isolation & purification
Sensitivity and Specificity
Viroporin Proteins / genetics*

Substances

Coronavirus Nucleocapsid Proteins
E protein, SARS coronavirus
N protein, SARS-CoV
RNA, Viral
Viroporin Proteins