Effect of high fat diet and excessive compressive mechanical force on pathologic changes of temporomandibular joint

Sci Rep. 2020 Oct 15;10(1):17457. doi: 10.1038/s41598-020-74326-z.

Abstract

The aim of this study was to investigate the effect of high fat diet and excessive compressive mechanical force on temporomandibular joint. In vivo, a mouse model of temporomandibular joint compressive loading device was used. A high fat diet mouse model and a combined mouse model intraperitoneally treated with or without simvastatin were used in the study. The pathological changes of mandibular condylar cartilage were assessed by Safranin-O staining. The IL-1β, MMP-3, leptin expression changes in the cartilage were detected by immunohistochemistry. In vitro, the mandibular condylar chondrocytes were treated with or without L-1β and simvastatin. The mRNA expression level of matrix MMPs and leptin were assessed. Both excessive compressive mechanical force and high fat diet induced obesity caused TMJ osteoarthritis-like changes and increased expression of IL-1β, MMP-3, and leptin. These pathological changes were much more serious when the two interventions were exerted together, while simvastatin could obviously alleviate these changes. The mRNA expression of MMP-3, MMP-13, and leptin increased in the IL-1β treated chondrocytes treated with IL-1β, and decreased with simvastatin treatment. The development of temporomandibular joint pathological changes could be caused by the excessive compressive mechanical force and high fat diet induced obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chondrocytes / metabolism
  • Diet, High-Fat*
  • Disease Models, Animal
  • Interleukin-1beta / metabolism*
  • Leptin / metabolism*
  • Male
  • Mandible / pathology
  • Matrix Metalloproteinase 3 / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Obesity
  • Phenazines / pharmacology
  • Risk Factors
  • Simvastatin / pharmacology
  • Stress, Mechanical*
  • Temporomandibular Joint / metabolism*
  • Temporomandibular Joint / physiopathology*

Substances

  • IL1B protein, mouse
  • Interleukin-1beta
  • Leptin
  • Phenazines
  • Simvastatin
  • Matrix Metalloproteinase 3
  • Mmp3 protein, mouse
  • safranine T