A Cytometrist's Guide to Coordinating and Performing Effective COVID-19 Research

Pratip K Chattopadhyay; Andrew Filby; Evan R Jellison; Guido Ferrari; Cherie Green; Sindhu Cherian; Jonathan Irish; Virginia Litwin; other members of the ISAC COVID-19 Work Group

doi:10.1002/cyto.a.24210

A Cytometrist's Guide to Coordinating and Performing Effective COVID-19 Research

Cytometry A. 2021 Jan;99(1):11-18. doi: 10.1002/cyto.a.24210. Epub 2020 Sep 8.

Authors

Pratip K Chattopadhyay¹, Andrew Filby², Evan R Jellison³, Guido Ferrari⁴, Cherie Green⁵, Sindhu Cherian⁶, Jonathan Irish⁷, Virginia Litwin⁸; other members of the ISAC COVID-19 Work Group

Affiliations

¹ Precision Immunology Laboratory, NYU Langone Health, New York, New York, USA.
² Flow Cytometry Core and Innovation, Methodology and Application Research Theme, Biosciences Institute, Newcastle University, Newcastle, UK.
³ Department of Immunology, UConn School of Medicine, Farmington, Connecticut, USA.
⁴ Department of Surgery - EQAPOL Flow Cytometry Program, Duke University Medical Center, Durham, North Carolina, USA.
⁵ Biomarker Development, Genentech/Roche, Oceanside, California, USA.
⁶ Department of Laboratory Medicine & Pathology, University of Washington, Seattle, Washington, USA.
⁷ Vanderbilt University, Nashville, Tennessee, USA.
⁸ Caprion Biosciences, Montreal, Quebec, Canada.

Abstract

Cytometry is playing a crucial role in addressing the COVID-19 pandemic. In this commentary-written by a variety of stakeholders in the cytometry, immunology, and infectious disease communities-we review cytometry's role in the COVID-19 response and discuss workflow issues critical to planning and executing effective research in this emerging field. We discuss sample procurement and processing, biosafety, technology options, data sharing, and the translation of research findings into clinical environments. © 2020 International Society for Advancement of Cytometry.

Keywords: COVID-19; SARS-CoV2; flow cytometry; immune monitoring; pandemic.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biomedical Research / methods
Biomedical Research / trends
COVID-19 / epidemiology
COVID-19 / prevention & control*
Containment of Biohazards / methods
Containment of Biohazards / trends*
Flow Cytometry / methods
Flow Cytometry / trends*
Humans
Information Dissemination / methods
SARS-CoV-2 / isolation & purification*
Translational Research, Biomedical / methods
Translational Research, Biomedical / trends*

Abstract

Publication types

MeSH terms

Grants and funding