Pancreatic cancers suppress negative feedback of glucose transport to reprogram chromatin for metastasis

Nat Commun. 2020 Aug 13;11(1):4055. doi: 10.1038/s41467-020-17839-5.

Abstract

Although metastasis is the most common cause of cancer deaths, metastasis-intrinsic dependencies remain largely uncharacterized. We previously reported that metastatic pancreatic cancers were dependent on the glucose-metabolizing enzyme phosphogluconate dehydrogenase (PGD). Surprisingly, PGD catalysis was constitutively elevated without activating mutations, suggesting a non-genetic basis for enhanced activity. Here we report a metabolic adaptation that stably activates PGD to reprogram metastatic chromatin. High PGD catalysis prevents transcriptional up-regulation of thioredoxin-interacting protein (TXNIP), a gene that negatively regulates glucose import. This allows glucose consumption rates to rise in support of PGD, while simultaneously facilitating epigenetic reprogramming through a glucose-fueled histone hyperacetylation pathway. Restoring TXNIP normalizes glucose consumption, lowers PGD catalysis, reverses hyperacetylation, represses malignant transcripts, and impairs metastatic tumorigenesis. We propose that PGD-driven suppression of TXNIP allows pancreatic cancers to avidly consume glucose. This renders PGD constitutively activated and enables metaboloepigenetic selection of additional traits that increase fitness along glucose-replete metastatic routes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / genetics
  • Biological Transport / physiology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cellular Reprogramming / genetics
  • Cellular Reprogramming / physiology
  • Chromatin / metabolism*
  • Chromatin Immunoprecipitation
  • Epigenesis, Genetic / genetics
  • Glucose / metabolism*
  • Mice
  • Mice, Nude
  • Pancreatic Neoplasms / genetics
  • Pancreatic Neoplasms / metabolism*
  • Pancreatic Neoplasms / pathology*
  • Phosphogluconate Dehydrogenase / genetics
  • Phosphogluconate Dehydrogenase / metabolism
  • Thioredoxins / genetics
  • Thioredoxins / metabolism

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • Chromatin
  • Txnip protein, mouse
  • Thioredoxins
  • Phosphogluconate Dehydrogenase
  • Glucose