Detection of community-acquired respiratory viruses in allogeneic stem-cell transplant recipients and controls-A prospective cohort study

Transpl Infect Dis. 2020 Dec;22(6):e13415. doi: 10.1111/tid.13415. Epub 2020 Jul 23.

Abstract

Background: Community-acquired respiratory viruses (CARV) cause upper and lower respiratory tract infections (URTI/LRTI) and may be life-threatening for recipients of an allogeneic stem cell transplantation (allo-SCT).

Methods: In a prospective study encompassing 4 winter-seasons, we collected throat gargles (TG) at random time points from allo-SCT recipients (patients) and controls and followed them up for at least 3 weeks including repetitive sampling and documentation of symptoms. A Multiplex-PCR system to identify 20 CARV and Mycoplasma pneumoniae was used to detect CARV.

Results: One hundred ninety-four patients with 426 TG and 273 controls with 549 TG were included. There were more patients with a positive test result (25% vs 11% in the controls), and the patients had a higher number of positive TG (70 = 16%) compared to controls (32 = 6%) (P < .001). Altogether, 115 viruses were detected. Multiple viruses in one TG (11/48, 34%) and prolonged shedding were only observed in patients (13/48, 27%). Patients had more RSV (18/83, 26%) and adenovirus (15/83, 21%) than controls (both viruses 2/32, 6%). Independent risk factors for the detection of CARV included age >40 years (OR 3.38, 95% CI 1.8-6.4, P < .001) and presence of URTI-symptoms (OR 3.22, 95% CI 1.9-5.5, P < .001). No controls developed a LRTI or died whereas 4/48 (8%) patients developed a LRTI (coronavirus in 2, RSV in 1 and influenza A H1N1 in 1 patient). One patient died of CARV (influenza A H1N1).

Conclusion: Allo-SCT-recipients have more CARV-infections, exhibit a different epidemiology, have more cases of co-infection or prolonged shedding and have a higher rate of LRTI and mortality.

Keywords: CARV; epidemiology; immunosuppression.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adenoviridae / isolation & purification
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Cohort Studies
  • Community-Acquired Infections / epidemiology
  • Community-Acquired Infections / etiology
  • Community-Acquired Infections / virology
  • Coronaviridae / isolation & purification
  • Female
  • Humans
  • Immunosuppression Therapy
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Male
  • Middle Aged
  • Mycoplasma pneumoniae / isolation & purification
  • Prospective Studies
  • Respiratory Syncytial Viruses / isolation & purification
  • Respiratory Tract Infections / epidemiology*
  • Respiratory Tract Infections / mortality
  • Respiratory Tract Infections / physiopathology
  • Respiratory Tract Infections / virology*
  • Risk Factors
  • Stem Cell Transplantation*
  • Transplant Recipients
  • Transplantation, Homologous
  • Virus Diseases / epidemiology*
  • Virus Diseases / mortality
  • Virus Diseases / physiopathology
  • Virus Diseases / virology*
  • Virus Shedding
  • Young Adult