MMP-9 Inhibitor GM6001 Prevents the Development of ssTBI-Induced Parkinson's Disease via the Autophagy Pathway

Cell Mol Neurobiol. 2021 Nov;41(8):1651-1663. doi: 10.1007/s10571-020-00933-z. Epub 2020 Aug 7.

Abstract

Concussion is a widely recognized environmental risk factor for neurodegenerative diseases, including Parkinson's disease (PD). Small-vessel disease of the brain has been reported to contribute to neurodegenerative diseases. In this study, we observed BBB disruption in wild-type (WT) mice, but not in matrix metalloproteinase 9 (MMP-9) knockout mice, subjected to single severe traumatic brain injury (ssTBI). Furthermore, treating ssTBI mice with the MMP-9 inhibitor GM6001 effectively maintained BBB integrity, promoted the elimination of damaged mitochondria via mitophagy, and then prevented neuronal death and progressive neurodegeneration. However, we did not observe this neuroprotective effect of MMP-9 inhibition in beclin-1-/+ mice. Collectively, these findings revealed that concussion led to BBB disruption via MMP-9, and that GM6001 prevented the development of PD via the autophagy pathway.

Keywords: Blood–brain barrier; MMP-9; Mitophagy; Parkinson’s disease; Traumatic brain injury.

MeSH terms

  • Animals
  • Autophagy / drug effects*
  • Autophagy / physiology
  • Brain / drug effects
  • Brain / enzymology
  • Brain / pathology
  • Brain Injuries, Traumatic / drug therapy*
  • Brain Injuries, Traumatic / enzymology
  • Brain Injuries, Traumatic / pathology
  • Dipeptides / pharmacology
  • Dipeptides / therapeutic use*
  • Female
  • Male
  • Matrix Metalloproteinase 9 / metabolism*
  • Matrix Metalloproteinase Inhibitors / pharmacology
  • Matrix Metalloproteinase Inhibitors / therapeutic use*
  • Maze Learning / drug effects
  • Maze Learning / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Parkinsonian Disorders / drug therapy*
  • Parkinsonian Disorders / enzymology
  • Parkinsonian Disorders / pathology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology
  • Trauma Severity Indices

Substances

  • Dipeptides
  • Matrix Metalloproteinase Inhibitors
  • N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide
  • Matrix Metalloproteinase 9
  • Mmp9 protein, mouse