Abstract
High-throughput screening methods have been used to identify two novel series of inhibitors that disrupt progranulin binding to sortilin. Exploration of structure-activity relationships (SAR) resulted in compounds with sufficient potency and physicochemical properties to enable co-crystallization with sortilin. These co-crystal structures supported observed SAR trends and provided guidance for additional avenues for designing compounds with additional interactions within the binding site.
Keywords:
Progranulin; Protein-protein interaction inhibitor; Sortilin; Structure activity relationship.
Copyright © 2020 Elsevier Ltd. All rights reserved.
MeSH terms
-
Adaptor Proteins, Vesicular Transport / antagonists & inhibitors
-
Adaptor Proteins, Vesicular Transport / metabolism*
-
Amides / chemistry
-
Amides / metabolism
-
Amino Acids / chemistry
-
Amino Acids / metabolism
-
Binding Sites
-
Crystallography, X-Ray
-
High-Throughput Screening Assays
-
Humans
-
Molecular Dynamics Simulation
-
Progranulins / antagonists & inhibitors
-
Progranulins / metabolism*
-
Protein Binding
-
Pyrazoles / chemistry
-
Pyrazoles / metabolism
-
Small Molecule Libraries / chemistry*
-
Small Molecule Libraries / metabolism
-
Structure-Activity Relationship
Substances
-
Adaptor Proteins, Vesicular Transport
-
Amides
-
Amino Acids
-
Progranulins
-
Pyrazoles
-
Small Molecule Libraries
-
sortilin