Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis

Juliana Felgueiras; Joana Vieira Silva; Alexandra Nunes; Inês Fernandes; António Patrício; Nuno Maia; Steven Pelech; Margarida Fardilha

doi:10.1016/j.jprot.2020.103888

Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis

J Proteomics. 2020 Aug 30:226:103888. doi: 10.1016/j.jprot.2020.103888. Epub 2020 Jun 30.

Authors

Juliana Felgueiras¹, Joana Vieira Silva², Alexandra Nunes³, Inês Fernandes³, António Patrício⁴, Nuno Maia⁴, Steven Pelech⁵, Margarida Fardilha⁶

Affiliations

¹ iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal; Cancer Biology and Epigenetics Group, IPO Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.
² iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal; i3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal; Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
³ iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal.
⁴ Centro Hospitalar do Baixo Vouga, EPE - Hospital Infante D. Pedro, 3814-501 Aveiro, Portugal.
⁵ Department of Medicine, University of British Columbia, Vancouver, B.C., Canada; Kinexus Bioinformatics Corporation, Suite 1 - 8755 Ash Street, Vancouver, B.C. V6P 6T3, Canada.
⁶ iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal. Electronic address: mfardilha@ua.pt.

PMID: 32619771
DOI: 10.1016/j.jprot.2020.103888

Abstract

Prostate cancer (PCa) treatment remains challenging, especially in advanced stages, where the lack of sensitivity and specificity of available biomarkers makes it difficult to establish an accurate prognosis. Therefore, it is imperative to study PCa biology to identify key molecules that can improve PCa management. In this study, eight prostate tumor tissues and paired normal tissues were analyzed using two approaches-Fourier-transform infrared (FT-IR) spectroscopy for spectroscopic profiling of biomolecules and antibody microarray for signaling proteins-with the main goal of identifying metabolic and proteomic changes that enable the distinction between normal and tumor conditions. Principal component analysis of FT-IR spectra revealed different spectroscopic signals for each condition. The most relevant changes in prostate tumor tissues identified by FT-IR were dysregulation in lipid metabolism, lower polysaccharide and glycogen content, higher nucleic acid content, and increased protein phosphorylation. Using an antibody microarray, 42 proteins were identified as differentially regulated between the two conditions; 14 of those revealed changes in their phosphorylation status. These proteins include transcription factors and kinases and constitute a highly-interconnected interaction network. Altogether, our data reveal metabolic and proteomic alterations that may be of interest in future translational studies aimed at establishing PCa prognosis and treatment. SIGNIFICANCE: Prostate tumor tissues and adjacent benign tissues were analyzed using two approaches-Fourier-transform infrared (FT-IR) spectroscopy for biomolecules and an antibody microarray for signaling proteins, which allowed to identify a panel of metabolic and proteomic alterations that may be of interest in future translational studies to enable the distinction between normal and tumor conditions.

Keywords: Fourier-transform infrared spectroscopy; Metabolomics; Microarray; Phosphorylation; Prostate cancer; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinogenesis
Humans
Male
Principal Component Analysis
Prostate*
Proteomics*
Spectroscopy, Fourier Transform Infrared